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Cammer, Michael-2 on
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I forgot to say that in the Plexiglas reply that this worked well as a model for cell cultures, but we are having an issue now (ok, in February and maybe again someday...) that as we focus deeper in drosophila embryos using a Bruker galvo miniscanner that there is a lot of scattering. At the surface of a zebrafish embryo we can target membranes specifically, but deeper in drosophila scatters too much for fine targeting. We have tried three different lenses and adjusting the collar of one empirically. This is at 405 and 470 nm. So spot size is very depth and sample dependent.
Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory
NYU Langone Health, 540 First Avenue, SK2 Microscopy Suite, New York, NY 10016
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From: Confocal Microscopy List <
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Sent: Thursday, April 23, 2020 6:21:30 PM
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Subject: Size of a bleach point
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Dear listers,
When using the Bleach Point feature in the Leica confocal systems (I'm sure
it also exists in the other brands), I don't know how to measure the
surface of the bleached area. I figure out the wavelength of the laser beam
and the NA of the lens affect the size of that area, so would it be correct
to use the resolution formula to calculate it? Or am I mixing up things
here?
Thank you very much,
Xavi.
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*Xavier Sanjuan*
Advanced Light Microscopy Unit
Parc de Recerca Biomèdica de Barcelona
Doctor Aiguader, 88
08003 Barcelona - Spain
Tel: + 34 93 316 0195 (ext 1195 dins el PRBB)
Fax: + 34 93 316 09 01
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