http://confocal-microscopy-list.275.s1.nabble.com/Size-of-a-bleach-point-tp7590808p7590815.html
del dia dv., 24 d’abr. 2020 a les 18:10:
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> I forgot to say that in the Plexiglas reply that this worked well as a
> model for cell cultures, but we are having an issue now (ok, in February
> and maybe again someday...) that as we focus deeper in drosophila embryos
> using a Bruker galvo miniscanner that there is a lot of scattering. At the
> surface of a zebrafish embryo we can target membranes specifically, but
> deeper in drosophila scatters too much for fine targeting. We have tried
> three different lenses and adjusting the collar of one empirically. This
> is at 405 and 470 nm. So spot size is very depth and sample dependent.
>
>
> Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory
>
> NYU Langone Health, 540 First Avenue, SK2 Microscopy Suite, New York, NY
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> ________________________________
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> Sent: Thursday, April 23, 2020 6:21:30 PM
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> Subject: Size of a bleach point
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> Dear listers,
>
> When using the Bleach Point feature in the Leica confocal systems (I'm sure
> it also exists in the other brands), I don't know how to measure the
> surface of the bleached area. I figure out the wavelength of the laser beam
> and the NA of the lens affect the size of that area, so would it be correct
> to use the resolution formula to calculate it? Or am I mixing up things
> here?
>
> Thank you very much,
>
> Xavi.
>
> ___________________________________
>
> *Xavier Sanjuan*
> Advanced Light Microscopy Unit
>
> Parc de Recerca Biomèdica de Barcelona
> Doctor Aiguader, 88
> 08003 Barcelona - Spain
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