Re: shopping: live-sample confocal+super-res

Posted by samuel connell on
URL: http://confocal-microscopy-list.275.s1.nabble.com/shopping-live-sample-confocal-super-res-tp7590816p7590818.html

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Hi Jeff,

*Commercial Reply*

You've chosen a solid group of instruments for this project. I'll drop you
an email off the list as well; we've been working with the SoRa as an early
adopter beta tester for more than two years now with 18 years of experience
in the Yokogawa SDC domain. We recently hosted a day of SoRa webinars a few
weeks ago. If anyone would like to view the one hour recorded version, let
me know and I'll send you a link.

Cheers,
-
Sam

Samuel Connell
Director of Sales

Intelligent Imaging Innovations (3i)
3575 Ringsby Ct, Suite 102
Denver, CO  80216  USA
1-720-437-6926
www.intelligent-imaging.com
[hidden email]


On Sun, Apr 26, 2020 at 12:16 PM Reece, Jeff (NIH/NIDDK) [E] <
[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear List,
>
> We are a core facility ready to make a major purchase, seeking advice.
> The system needs to provide fast, live-sample confocal imaging, but also
> super-res in the 100-150nm range (xy).  Here is a sampling of the
> applications we are trying to satisfy:
>
>   1.  Z-stacks of cultured cells over time, multi-color labeled.
> Super-res and standard confocal.
>   2.  Z-stacks and/or time series of live tissue/organisms (e.g. c.
> elegans, oocytes) up to 40 microns deep (at least), multi-color labeled,
> super-res and standard confocal.
>   3.  Z-stack, tile and stitch, super-res of fixed samples, e.g. FISH and
> tissue slices (e.g. mouse kidney).
>
>
>
> We narrowed it down to the following instruments:
>
>   1.  Nikon W1 SoRa spinning disk
>   2.  Olympus W1 SoRa spinning disk ("SpinSR")
>   3.  Visitech vt-iSIM (VisiView software seems to be the best choice here
> in the USA?)
>   4.  Zeiss LSM 980 AiryScan 2
>   5.  Zeiss Elyra 7 Lattice SIM
>
>
> I will send another email for those that are theoretical-minded; for this
> email, I am interested in practical, hands-on impressions.
> For any of you that have compared any of the above systems, I would
> greatly appreciate to hear those impressions, either to the list or
> directly to me.
> Here are some common categories of comparison that may jog your memory
> and/or provide a framework for your response:
>
>   1.  Resolution;
>   2.  Speed;
>   3.  Sensitivity;
>   4.  Photobleaching;
>   5.  Maintaining focal plane over time (all the vendors do this well
> now?);
>   6.  Color-correction from blue to far red, to edge of image field;
>   7.  Usability of software - i.e. user-friendliness, appropriate for a
> core facility;
>   8.  Functionality-- i.e. range of features; capability to do what you
> need from a workflow/experimental point of view;
>   9.  Reliability, robustness of the system;
>   10. Customer support level.
>
> Stay Safe and Healthy,
> Jeff
>
> Jeff Reece
> Ph: +1.301.451.4330
> E:  [hidden email]<
> https://mail.nih.gov/owa/14.3.174.1/scripts/premium/redir.aspx?C=vorg2hwQ3EG79HF4VARC2_-txi1AZNEITAaQhKx2WUBLeDOG3BM2dSsWeRsCBbyhbstXsPzU2G8.&URL=mailto%3ajeff.reece%40nih.gov
> >
>
> Director, Advanced Light Microscopy & Image Analysis Core (ALMIAC)
> NIH (National Institutes of Health) /
>    NIDDK (National Institute of Diabetes and Digestive and Kidney diseases)
> 8 Center Dr, Rm 126
> Bethesda, MD
> 20892-0851
>
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