Posted by
Csúcs Gábor-3 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/shopping-live-sample-confocal-super-res-tp7590816p7590834.html
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Dear Jeff,
Some very short/minor points to be considered:
1) We have both techniques - for me SIM and AiryScan (SoRa) play in a different league in terms of resolution.
2) I believe that in the mean time you can run the vt-iSIM also with Nikon NIS Elements (which for me is a better option than VisiView). Of course this assumes that you go with a Nikon microscope body.
3) You may want to look also at the "Re-scan" technology (represented by
http://www.confocal.nl/). This is a very powerful and also economic solution. We are also planning to invest in a SoRa and my idea is to have on the same system also a Re-Scan setup... If you buy an appropriate laser box (with multiple outputs), this is a relatively small additional cost..
Greetings Gabor
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From: Confocal Microscopy List <
[hidden email]> On Behalf Of Reece, Jeff (NIH/NIDDK) [E]
Sent: Sunday, April 26, 2020 9:07 PM
To:
[hidden email]
Subject: shopping: live-sample confocal+super-res
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Dear List,
We are a core facility ready to make a major purchase, seeking advice. The system needs to provide fast, live-sample confocal imaging, but also super-res in the 100-150nm range (xy). Here is a sampling of the applications we are trying to satisfy:
1. Z-stacks of cultured cells over time, multi-color labeled. Super-res and standard confocal.
2. Z-stacks and/or time series of live tissue/organisms (e.g. c. elegans, oocytes) up to 40 microns deep (at least), multi-color labeled, super-res and standard confocal.
3. Z-stack, tile and stitch, super-res of fixed samples, e.g. FISH and tissue slices (e.g. mouse kidney).
We narrowed it down to the following instruments:
1. Nikon W1 SoRa spinning disk
2. Olympus W1 SoRa spinning disk ("SpinSR")
3. Visitech vt-iSIM (VisiView software seems to be the best choice here in the USA?)
4. Zeiss LSM 980 AiryScan 2
5. Zeiss Elyra 7 Lattice SIM
I will send another email for those that are theoretical-minded; for this email, I am interested in practical, hands-on impressions.
For any of you that have compared any of the above systems, I would greatly appreciate to hear those impressions, either to the list or directly to me.
Here are some common categories of comparison that may jog your memory and/or provide a framework for your response:
1. Resolution;
2. Speed;
3. Sensitivity;
4. Photobleaching;
5. Maintaining focal plane over time (all the vendors do this well now?);
6. Color-correction from blue to far red, to edge of image field;
7. Usability of software - i.e. user-friendliness, appropriate for a core facility;
8. Functionality-- i.e. range of features; capability to do what you need from a workflow/experimental point of view;
9. Reliability, robustness of the system;
10. Customer support level.
Stay Safe and Healthy,
Jeff
Jeff Reece
Ph: +1.301.451.4330
E:
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