Posted by
James Kerin on
URL: http://confocal-microscopy-list.275.s1.nabble.com/shopping-live-sample-confocal-super-res-tp7590816p7590842.html
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Dear Jeff,
If you are considering spinning disk configurations with super-resolution capability then another combination to consider is the Crest X-light v3 spinning disk confocal together with the Gataca Live-SR. The v3 of the X-light is a great and cost effective low light confocal imager, couple this with the equally cost-effective Live-SR for the optical modulation to achieve super-resolution imaging. The combo will take you down to around 120nm resolution.
This recommendation is made from an honest belief that you should take a look and give serious consideration, but please note I have to declare a commercial interest as Cairn use the Crest X-light and Gataca LiveSR in our configurations in the UK and Europe.
https://crestopt.com/xlightv3/https://www.gataca-systems.com/Martyn (on James' email account)
Dr Martyn Reynolds
Head of Advanced Imaging
Direct: + 44 (0)7795 304090
On 04/26/2020, 08:06pm, "Reece, Jeff (NIH/NIDDK) [E]" (
[hidden email]) wrote:
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Dear List,
We are a core facility ready to make a major purchase, seeking advice. The system needs to provide fast, live-sample confocal imaging, but also super-res in the 100-150nm range (xy). Here is a sampling of the applications we are trying to satisfy:
1. Z-stacks of cultured cells over time, multi-color labeled. Super-res and standard confocal.
2. Z-stacks and/or time series of live tissue/organisms (e.g. c. elegans, oocytes) up to 40 microns deep (at least), multi-color labeled, super-res and standard confocal.
3. Z-stack, tile and stitch, super-res of fixed samples, e.g. FISH and tissue slices (e.g. mouse kidney).
We narrowed it down to the following instruments:
1. Nikon W1 SoRa spinning disk
2. Olympus W1 SoRa spinning disk ("SpinSR")
3. Visitech vt-iSIM (VisiView software seems to be the best choice here in the USA?)
4. Zeiss LSM 980 AiryScan 2
5. Zeiss Elyra 7 Lattice SIM
I will send another email for those that are theoretical-minded; for this email, I am interested in practical, hands-on impressions.
For any of you that have compared any of the above systems, I would greatly appreciate to hear those impressions, either to the list or directly to me.
Here are some common categories of comparison that may jog your memory and/or provide a framework for your response:
1. Resolution;
2. Speed;
3. Sensitivity;
4. Photobleaching;
5. Maintaining focal plane over time (all the vendors do this well now?);
6. Color-correction from blue to far red, to edge of image field;
7. Usability of software - i.e. user-friendliness, appropriate for a core facility;
8. Functionality-- i.e. range of features; capability to do what you need from a workflow/experimental point of view;
9. Reliability, robustness of the system;
10. Customer support level.
Stay Safe and Healthy,
Jeff
Jeff Reece
Ph: +1.301.451.4330
E:
[hidden email]<
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Director, Advanced Light Microscopy & Image Analysis Core (ALMIAC)
NIH (National Institutes of Health) /
NIDDK (National Institute of Diabetes and Digestive and Kidney diseases)
8 Center Dr, Rm 126
Bethesda, MD
20892-0851
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