Confocal Microscopy List - Re: shopping: live-sample confocal+super-res

Re: shopping: live-sample confocal+super-res

Posted by Cammer, Michael-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/shopping-live-sample-confocal-super-res-tp7590816p7590843.html

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Has anyone mentioned yet that if anyone needs to do localized photoactivation, bleaching, ablation, with spinning disk or ISIM you need an additional unit like the Bruker miniscanner with additional lasers and matching filter blocks etc?

Laser scanning confocal may be better for this.

But the white light laser for Leica, which otherwise is an incredible confocal, may not be strong enough?

For most live work, ISIM or spinning disk way better. Exception would be for yeast and bacteria where we like to zoom in and over sample.

Cheers-

Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory

NYU Langone Health, 540 First Avenue, SK2 Microscopy Suite, New York, NY 10016

[hidden email]<mailto:[hidden email]> http://nyulmc.org/micros http://microscopynotes.com/

Voice direct only, no text or messages: 1-914-309-3270 and 1-646-501-0567

________________________________
From: Confocal Microscopy List <[hidden email]> on behalf of James Kerin <[hidden email]>
Sent: Tuesday, April 28, 2020 10:39 AM
To: [hidden email]
Subject: Re: shopping: live-sample confocal+super-res

[EXTERNAL]

*****
To join, leave or search the confocal microscopy listserv, go to:
https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIFAw&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=VGygx11l5BTnh0tin0WYmhlIVJwDAlMSr74ybuxIzUE&s=gFKMwrwsO8fSWjZUweTO-6pdQv6nLjLNXb377YvAxOU&e=
Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIFAw&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=VGygx11l5BTnh0tin0WYmhlIVJwDAlMSr74ybuxIzUE&s=dTYmXyUhHNfpUWhYBbjAA5v_cbCAOV0XRjdZP0RO76Q&e= and include the link in your posting.
*****

 ***** COMMERCIAL POST *****

Dear Jeff,

If you are considering spinning disk configurations with super-resolution capability then another combination to consider is the Crest X-light v3 spinning disk confocal together with the Gataca Live-SR. The v3 of the X-light is a great and cost effective low light confocal imager, couple this with the equally cost-effective Live-SR for the optical modulation to achieve super-resolution imaging. The combo will take you down to around 120nm resolution.
This recommendation is made from an honest belief that you should take a look and give serious consideration, but please note I have to declare a commercial interest as Cairn use the Crest X-light and Gataca LiveSR in our configurations in the UK and Europe.

https://urldefense.proofpoint.com/v2/url?u=https-3A__crestopt.com_xlightv3_&d=DwIFAw&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=VGygx11l5BTnh0tin0WYmhlIVJwDAlMSr74ybuxIzUE&s=c3vRjzCudBMPGVJI2zA3jz6uv94ANofm-F10pXNVEOs&e=

https://urldefense.proofpoint.com/v2/url?u=https-3A__www.gataca-2Dsystems.com_&d=DwIFAw&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=VGygx11l5BTnh0tin0WYmhlIVJwDAlMSr74ybuxIzUE&s=kaf25E_h6snd0qfol8M6DgwhvSCiE2G3HtBDQ_zkvK0&e=

Martyn (on James' email account)

Dr Martyn Reynolds
Head of Advanced Imaging
Direct: + 44 (0)7795 304090
 

 
 

 
 
 
 
 

On 04/26/2020, 08:06pm, "Reece, Jeff (NIH/NIDDK) [E]" ([hidden email]) wrote:
*****

To join, leave or search the confocal microscopy listserv, go to:

https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIFAw&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=VGygx11l5BTnh0tin0WYmhlIVJwDAlMSr74ybuxIzUE&s=gFKMwrwsO8fSWjZUweTO-6pdQv6nLjLNXb377YvAxOU&e=

Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIFAw&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=VGygx11l5BTnh0tin0WYmhlIVJwDAlMSr74ybuxIzUE&s=dTYmXyUhHNfpUWhYBbjAA5v_cbCAOV0XRjdZP0RO76Q&e= and include the link in your posting.

*****

Dear List,

We are a core facility ready to make a major purchase, seeking advice. The system needs to provide fast, live-sample confocal imaging, but also super-res in the 100-150nm range (xy). Here is a sampling of the applications we are trying to satisfy:

1. Z-stacks of cultured cells over time, multi-color labeled. Super-res and standard confocal.

2. Z-stacks and/or time series of live tissue/organisms (e.g. c. elegans, oocytes) up to 40 microns deep (at least), multi-color labeled, super-res and standard confocal.

3. Z-stack, tile and stitch, super-res of fixed samples, e.g. FISH and tissue slices (e.g. mouse kidney).

We narrowed it down to the following instruments:

1. Nikon W1 SoRa spinning disk

2. Olympus W1 SoRa spinning disk ("SpinSR")

3. Visitech vt-iSIM (VisiView software seems to be the best choice here in the USA?)

4. Zeiss LSM 980 AiryScan 2

5. Zeiss Elyra 7 Lattice SIM

I will send another email for those that are theoretical-minded; for this email, I am interested in practical, hands-on impressions.

For any of you that have compared any of the above systems, I would greatly appreciate to hear those impressions, either to the list or directly to me.

Here are some common categories of comparison that may jog your memory and/or provide a framework for your response:

1. Resolution;

2. Speed;

3. Sensitivity;

4. Photobleaching;

5. Maintaining focal plane over time (all the vendors do this well now?);

6. Color-correction from blue to far red, to edge of image field;

7. Usability of software - i.e. user-friendliness, appropriate for a core facility;

8. Functionality-- i.e. range of features; capability to do what you need from a workflow/experimental point of view;

9. Reliability, robustness of the system;

10. Customer support level.

Stay Safe and Healthy,

Jeff

Jeff Reece

Ph: +1.301.451.4330

E: [hidden email]<https://urldefense.proofpoint.com/v2/url?u=https-3A__mail.nih.gov_owa_14.3.174.1_scripts_premium_redir.aspx-3FC-3Dvorg2hwQ3EG79HF4VARC2-5F-2Dtxi1AZNEITAaQhKx2WUBLeDOG3BM2dSsWeRsCBbyhbstXsPzU2G8.-26URL-3Dmailto-253ajeff.reece-2540nih.gov-26gt-3B&d=DwIFAw&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=VGygx11l5BTnh0tin0WYmhlIVJwDAlMSr74ybuxIzUE&s=qH5QjNZue103aUFQe1A_Ud03OABkPuLGTmi5q-mM7_A&e=

Director, Advanced Light Microscopy & Image Analysis Core (ALMIAC)

NIH (National Institutes of Health) /

NIDDK (National Institute of Diabetes and Digestive and Kidney diseases)

8 Center Dr, Rm 126

Bethesda, MD

20892-0851

NOTE: THIS EMAIL IS CONFIDENTIAL, FOR THE ADDRESSED AUDIENCE ONLY UNLESS OTHERWISE SPECIFIED