http://confocal-microscopy-list.275.s1.nabble.com/organoid-imaging-tp7590835p7590855.html
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> Hello Mary Ellen,
>
> We had an interesting talk by Daniel Fulton from the University of
> Birmingham, UK (
>
https://www.birmingham.ac.uk/staff/profiles/inflammation-ageing/fulton-daniel.aspx> )
>
> at our recent on-line conference, where he spoke about long term live
> imaging of oligodendrocyte myelination in organotypic brain slice cultures.
> Maybe some of his techniques might be useful?
>
> His talk is at the end of the session located at this link:
>
>
https://www.youtube.com/watch?v=0L7tDQ2vDsM>
>
> Best regards
>
> Phillipa
>
> Dr Phillipa Timmins
> Head of Sales
>
> Aurox Ltd
> Culham Science Centre
> Abingdon
> Oxfordshire
> OX14 3DB
>
> Tel: 07585 676763
>
>
>
> -----Original Message-----
> From: Confocal Microscopy List <
[hidden email]> On
> Behalf Of Mary Ellen Pease
> Sent: 28 April 2020 15:22
> To:
[hidden email]
> Subject: organoid imaging
>
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>
> Hi all,
>
> I have a user who would like to image live organoids via
> confocal/multiphoton without jeopardizing the sample. Has anyone figured
> out a material in which the organoid could be placed that would eliminate
> movement/drift during imaging, without damaging it so the sample could go
> back into culture afterwards? So far, the papers I've looked at on this
> topic cryoembed them at a certain time point for immunohistochemistry. My
> user's lab has samples which are expressing GFP and RFP, and would like to
> look at them in vivo. Someone in our department suggested putting them in a
> mix of matrigel and culture media, which could slow them down temporarily,
> but in my experience, matrigel attenuates the signal. We have a multiphoton
> system on an inverted Zeiss platform (LSM 710). we can image using an
> objective inverter and dipping lenses, or from the bottom through a
> coverglass dish. Since we are working remotely at this point in time, I was
> hoping to get some direction from this group.
>
> Best,
>
> Mary Ellen Pease
> Manager, Wilmer Microscopy and Imaging Core Facility (MICF) Johns Hopkins
> SOM, Baltimore MD
>