Re: Phase Contrast Microscopy ... defense of brightfield microscopy

Posted by Catalin Chiritescu on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Phase-Contrast-Microscopy-tp7590859p7590877.html

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

You lose about 20-30% of light when a Phase Contrast objective is used in fluorescence. AFAIK Nikon Ti (don't know about TI2) has an external phase ring setup that you can use for Phase Contrast imaging with BF objectives - you can take it out of fluorescence light path to avoid the loss and PSF disruption. We considered that option for our Quantitative Phase Imaging SLIM systems.