Re: Phase Contrast Microscopy ... defense of brightfield microscopy
Posted by
Knecht, David on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Phase-Contrast-Microscopy-tp7590859p7590880.html
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I suspect the higher number (20%) is if you consider loss in both excitation and emission through the phase objective, but in the excitation path, you usually have plenty of extra power to compensate for a bit of loss in excitation efficiency. So I think the lower number is more realistic.
Dr. David Knecht
Professor, Department of Molecular and Cell Biology
University of Connecticut
91 N. Eagleville Rd.
U-3125
Storrs, CT 06269-3125
On May 4, 2020, at 2:20 AM, Catalin Chiritescu <
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*Message sent from a system outside of UConn.*
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You lose about 20-30% of light when a Phase Contrast objective is used in fluorescence. AFAIK Nikon Ti (don't know about TI2) has an external phase ring setup that you can use for Phase Contrast imaging with BF objectives - you can take it out of fluorescence light path to avoid the loss and PSF disruption. We considered that option for our Quantitative Phase Imaging SLIM systems.