Mounting tissue section onto the coverslip

Posted by Erika Wee-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Mounting-tissue-section-onto-the-coverslip-tp7591021.html

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Hello,

When I train people here to use the microscopes in the facility, 90% of the samples the tissue sections were mounted on the glass slide with lots of bubbles, so I am trying to convince people to mount the tissue section directly onto the cover-slip side.

But many people told me the tissue won't stay stick on the cover-slips. Would you mind to share how you or your lab mount the tissues sections? Do you or your histology core mount tissues directly onto the coverslips? Do you charge or coat the coverslips so the tissue section can stick better for staining?

Any information would be greatly appreciated.

Many thanks.
Best,
Erika