Re: Mounting tissue section onto the coverslip

Posted by Rhonda Powell on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Mounting-tissue-section-onto-the-coverslip-tp7591021p7591022.html

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Hi Erika,

Having done a little bit of histology myself, I can say that it’s a lot easier said than done to get tissue sections cut and placed on a cover slip!

If they have issues with bubbles, I feel like that would be true whether they place the tissue on the slide or the coverslip though.

Is your bubble issue with cryosections or paraffin sections? That might help some of us with limited histology experience give some other troubleshooting advice.

I also have some histo friends I could refer your question to if we can’t help!

Rhonda

Rhonda Reigers Powell
Research Lab Manager
Clemson Light Imaging Facility
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From: Confocal Microscopy List <[hidden email]> on behalf of Erika Wee <[hidden email]>
Sent: Monday, June 22, 2020 2:45 PM
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Subject: Mounting tissue section onto the coverslip

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Hello,

When I train people here to use the microscopes in the facility, 90% of the samples the tissue sections were mounted on the glass slide with lots of bubbles, so I am trying to convince people to mount the tissue section directly onto the cover-slip side.

But many people told me the tissue won't stay stick on the cover-slips. Would you mind to share how you or your lab mount the tissues sections? Do you or your histology core mount tissues directly onto the coverslips? Do you charge or coat the coverslips so the tissue section can stick better for staining?

Any information would be greatly appreciated.

Many thanks.
Best,
Erika