Re: Mounting tissue section onto the coverslip

Posted by Benjamin Smith on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Mounting-tissue-section-onto-the-coverslip-tp7591021p7591023.html

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I can say the trick for me has always been to put a small drop of
mounting media on top of the sample on the slide, and then using a pair of
#5 forceps (or any ultrafine forcep) holding onto one side of the
coverslip.  Holding the coverslip vertically relative to the slide, lower
the coverslip until the side opposite the forceps is resting on the slide,
and then gently lower the side with the forceps until the media first wets
onto the coverslip.  Continue to gently lower the coverslip, allowing the
mounting media to wick along the coverslip.  This way, no air will ever get
trapped under the coverslip.  In short, gradually lower just one side of
the coverslip so that the mounting media can wet along the length of the
coverslip, and you will almost never get air bubbles.

Cheers,
   Ben Smith

On Mon, Jun 22, 2020 at 11:45 AM Erika Wee <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hello,
>
> When I train people here to use the microscopes in the facility, 90% of
> the samples the tissue sections were mounted on the glass slide with lots
> of bubbles, so I am trying to convince people to mount the tissue section
> directly onto the cover-slip side.
>
> But many people told me the tissue won't stay stick on the cover-slips.
> Would you mind to share how you or your lab mount the tissues sections? Do
> you or your histology core mount tissues directly onto the coverslips? Do
> you charge or coat the coverslips so the tissue section can stick better
> for staining?
>
> Any information would be greatly appreciated.
>
> Many thanks.
> Best,
> Erika
>

--
Benjamin E. Smith, Ph. D.
Imaging Specialist, Vision Science
University of California, Berkeley
195 Life Sciences Addition
Berkeley, CA  94720-3200
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Fax [phone]
e-mail: [hidden email]
https://vision.berkeley.edu/faculty/core-grants-nei/core-grant-microscopic-imaging/