Re: Mounting tissue section onto the coverslip

Posted by Erika Wee-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Mounting-tissue-section-onto-the-coverslip-tp7591021p7591024.html

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi Rhonda,

Thanks very much for your quick response.

Our histology core usually mount sessions onto the glass-slides for the labs, which I can understand, and they were sectioned and mounted beautifully, but the students who was working on post staining and mounting, they use big cover-slip to cover many tissue sections and put uneven mounting media in between, also you can see there are bubbles everywhere.

Some labs here are doing their own cryosections in lab, and they told me the cryosectioned tissue won't stay onto the coverslips.

This is fine if they are using 10x or 20x to do whole section imaging, but for RNA-FISH or for higher resolution imaging, mounting the section to the coverslip become more critical.  

Best,
Erika