Re: Mounting tissue section onto the coverslip

Posted by Craig Brideau on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Mounting-tissue-section-onto-the-coverslip-tp7591021p7591025.html

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Could this be due to the slides being charged, while the coverslips are
not? Most of the slides I have used are charged to encourage the tissue to
stick. Otherwise some sort of surface preparation or pre-coating may be
necessary to promote adhesion.

Craig

On Mon, Jun 22, 2020 at 2:05 PM Erika Wee <[hidden email]> wrote:

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>
> Hi Rhonda,
>
> Thanks very much for your quick response.
>
> Our histology core usually mount sessions onto the glass-slides for the
> labs, which I can understand, and they were sectioned and mounted
> beautifully, but the students who was working on post staining and
> mounting, they use big cover-slip to cover many tissue sections and put
> uneven mounting media in between, also you can see there are bubbles
> everywhere.
>
> Some labs here are doing their own cryosections in lab, and they told me
> the cryosectioned tissue won't stay onto the coverslips.
>
> This is fine if they are using 10x or 20x to do whole section imaging, but
> for RNA-FISH or for higher resolution imaging, mounting the section to the
> coverslip become more critical.
>
> Best,
> Erika
>