Re: [EXTERNAL] Re: Mounting tissue section onto the coverslip

Posted by Julian Smith III on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Mounting-tissue-section-onto-the-coverslip-tp7591021p7591027.html

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Thanks, Ben
Exactly the method I've been using for over four decades now.
One addition: if you have giant sections (think whole rat heart), or a
half-dozen rows of serial sections, put a drop of clearing agent on the
section, then add the drop of mounting medium. Depending on how hot your
lab is (or if you're working in a hood), the clearing agent (I use
xylene) can partially evaporate before you get the coverslip down.
Julian

On 6/22/20 3:23 PM, Benjamin Smith wrote:

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>
> I can say the trick for me has always been to put a small drop of
> mounting media on top of the sample on the slide, and then using a pair of
> #5 forceps (or any ultrafine forcep) holding onto one side of the
> coverslip.  Holding the coverslip vertically relative to the slide, lower
> the coverslip until the side opposite the forceps is resting on the slide,
> and then gently lower the side with the forceps until the media first wets
> onto the coverslip.  Continue to gently lower the coverslip, allowing the
> mounting media to wick along the coverslip.  This way, no air will ever get
> trapped under the coverslip.  In short, gradually lower just one side of
> the coverslip so that the mounting media can wet along the length of the
> coverslip, and you will almost never get air bubbles.
>
> Cheers,
>     Ben Smith
>
> On Mon, Jun 22, 2020 at 11:45 AM Erika Wee <[hidden email]> wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> Post images on http://www.imgur.com and include the link in your posting.
>> *****
>>
>> Hello,
>>
>> When I train people here to use the microscopes in the facility, 90% of
>> the samples the tissue sections were mounted on the glass slide with lots
>> of bubbles, so I am trying to convince people to mount the tissue section
>> directly onto the cover-slip side.
>>
>> But many people told me the tissue won't stay stick on the cover-slips.
>> Would you mind to share how you or your lab mount the tissues sections? Do
>> you or your histology core mount tissues directly onto the coverslips? Do
>> you charge or coat the coverslips so the tissue section can stick better
>> for staining?
>>
>> Any information would be greatly appreciated.
>>
>> Many thanks.
>> Best,
>> Erika
>>
>
> --
> Benjamin E. Smith, Ph. D.
> Imaging Specialist, Vision Science
> University of California, Berkeley
> 195 Life Sciences Addition
> Berkeley, CA  94720-3200
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--
Julian P.S. Smith III
Director, Winthrop Microscopy Facility
Dept. of Biology
Winthrop University
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