Posted by
Tobias Baskin on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Mounting-tissue-section-onto-the-coverslip-tp7591021p7591028.html
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Hi Erika,
If you have access to a plasma cleaner putting your slides
with sections in a plasma for 30 sec or so just before mounting makes
the slides and sections super hydrophyllic and the mounting medium wets
a treat. I found this trick quite helpful for minimizing bubbles, esp
those that love to form right over the section. Hope this helps. Tobias
On 6/22/20 2:42 PM, Erika Wee wrote:
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>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> Post images on
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>
> Hello,
>
> When I train people here to use the microscopes in the facility, 90% of the samples the tissue sections were mounted on the glass slide with lots of bubbles, so I am trying to convince people to mount the tissue section directly onto the cover-slip side.
>
> But many people told me the tissue won't stay stick on the cover-slips. Would you mind to share how you or your lab mount the tissues sections? Do you or your histology core mount tissues directly onto the coverslips? Do you charge or coat the coverslips so the tissue section can stick better for staining?
>
> Any information would be greatly appreciated.
>
> Many thanks.
> Best,
> Erika
--
__ ___ ^ ___ ___ Tobias I. Baskin
/ \ / / \ / \ Professor (he)
/ / / / \ \ \ Biology Department
/ __/ /__ /___ \ \ \__ University of Mass.
/ / / \ \ \ 611 N. Pleasant St.
/ / / \ \ \ Amherst, Massachusetts
/ /___ / \ \___/ \_____ USA 01003 413-545-1533
www.bio.umass.edu/biology/baskin BLOG: blogs.umass.edu/baskin/