Confocal Microscopy List - Re: Mounting tissue section onto the coverslip

Re: Mounting tissue section onto the coverslip

Posted by Caroline Miller-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Mounting-tissue-section-onto-the-coverslip-tp7591021p7591037.html

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

You will still get bubbles, just on the underneath if you put the sections on the coverslip.

I put the coverslip flat on kimwipe, then a dot of moutant on the coverslip (you really need to play with the right amount for your application), then wet the moutant with a drop of liquid (either xylene based or PBS, again depending on application), then flip the slide and catch the drop on the slide and let capillary action do the rest.

You can push out small bubbles, but use a tool, not your greasy fingers or moutant smeared gloves, I use a metal probe.

I then wick up the excess with a kimwipe on the edge of the slide

Important! Don't keep smearing the moutant, just start again if it looks like a mess.

Remember smears are the enemy of good imaging!

Caroline

Caroline Miller
Associate Director of Immunohistochemistry and Analytical Histology
Unity Biotechnology

________________________________
From: Confocal Microscopy List <[hidden email]> on behalf of Tobias Baskin <[hidden email]>
Sent: Monday, June 22, 2020 1:54:56 PM
To: [hidden email] <[hidden email]>
Subject: Re: Mounting tissue section onto the coverslip

*****
To join, leave or search the confocal microscopy listserv, go to:
https://nam03.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.umn.edu%2Fcgi-bin%2Fwa%3FA0%3Dconfocalmicroscopy&data=02%7C01%7Ccaroline.miller%40UNITYBIOTECHNOLOGY.COM%7C6ad7b76ef75844a7ef0d08d816f73dcf%7Ce8d0e2ada88a4f3b8d7728bebd91e4a6%7C0%7C0%7C637284598443495373&sdata=G8ZgNyYpced3A7yeFkM0NTUDWRlh0bpyq1G8FLOWI1c%3D&reserved=0
Post images on https://nam03.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.imgur.com%2F&data=02%7C01%7Ccaroline.miller%40UNITYBIOTECHNOLOGY.COM%7C6ad7b76ef75844a7ef0d08d816f73dcf%7Ce8d0e2ada88a4f3b8d7728bebd91e4a6%7C0%7C0%7C637284598443495373&sdata=Ef92sudfzyPfXcH3tuGL8Oi9Tz7YIHqi2OP2LGDe79A%3D&reserved=0 and include the link in your posting.
*****

Hi Erika,
If you have access to a plasma cleaner putting your slides
with sections in a plasma for 30 sec or so just before mounting makes
the slides and sections super hydrophyllic and the mounting medium wets
a treat. I found this trick quite helpful for minimizing bubbles, esp
those that love to form right over the section. Hope this helps. Tobias

On 6/22/20 2:42 PM, Erika Wee wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> https://nam03.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.umn.edu%2Fcgi-bin%2Fwa%3FA0%3Dconfocalmicroscopy&data=02%7C01%7Ccaroline.miller%40UNITYBIOTECHNOLOGY.COM%7C6ad7b76ef75844a7ef0d08d816f73dcf%7Ce8d0e2ada88a4f3b8d7728bebd91e4a6%7C0%7C0%7C637284598443495373&sdata=G8ZgNyYpced3A7yeFkM0NTUDWRlh0bpyq1G8FLOWI1c%3D&reserved=0
> Post images on https://nam03.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.imgur.com%2F&data=02%7C01%7Ccaroline.miller%40UNITYBIOTECHNOLOGY.COM%7C6ad7b76ef75844a7ef0d08d816f73dcf%7Ce8d0e2ada88a4f3b8d7728bebd91e4a6%7C0%7C0%7C637284598443495373&sdata=Ef92sudfzyPfXcH3tuGL8Oi9Tz7YIHqi2OP2LGDe79A%3D&reserved=0 and include the link in your posting.
> *****
>
> Hello,
>
> When I train people here to use the microscopes in the facility, 90% of the samples the tissue sections were mounted on the glass slide with lots of bubbles, so I am trying to convince people to mount the tissue section directly onto the cover-slip side.
>
> But many people told me the tissue won't stay stick on the cover-slips. Would you mind to share how you or your lab mount the tissues sections? Do you or your histology core mount tissues directly onto the coverslips? Do you charge or coat the coverslips so the tissue section can stick better for staining?
>
> Any information would be greatly appreciated.
>
> Many thanks.
> Best,
> Erika

--
__ ___ ^ ___ ___ Tobias I. Baskin
/ \ / / \ / \ Professor (he)
/ / / / \ \ \ Biology Department
/ __/ /__ /___ \ \ \__ University of Mass.
/ / / \ \ \ 611 N. Pleasant St.
/ / / \ \ \ Amherst, Massachusetts
/ /___ / \ \___/ \_____ USA 01003 413-545-1533
https://nam03.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.bio.umass.edu%2Fbiology%2Fbaskin&data=02%7C01%7Ccaroline.miller%40UNITYBIOTECHNOLOGY.COM%7C6ad7b76ef75844a7ef0d08d816f73dcf%7Ce8d0e2ada88a4f3b8d7728bebd91e4a6%7C0%7C0%7C637284598443505369&sdata=rqoQfafFXbUd5uB6erXymbyoyK4RpMszSErNiPZwd%2BY%3D&reserved=0 BLOG: blogs.umass.edu/baskin/
CONFIDENTIAL - This message and the attached files may contain information that is confidential or proprietary. You are required to safeguard and restrict the use of confidential information in compliance with your obligations to Unity Biotechnology. If you are not the intended recipient, you may not duplicate, distribute, disclose, or otherwise make use of this message, the attachments, or any of the information it contains. Please notify the sender by return email and delete all copies of this message and the attachments.