Re: Mounting tissue section onto the coverslip

Posted by Sylvie Le Guyader on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Mounting-tissue-section-onto-the-coverslip-tp7591021p7591039.html

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About wrinkly sections:
This takes practice at the microtome.
But in general I talk people out of making super thin sections. I think that making sections as thin as possible is a remnant of hospital practice where one uses bright field images and colour dyes.
I advise people to make sections of over 1 cell diameter so that they do not cut every single cell. It is rare that the scientific question requires cutting every single cell.
Having slightly thicker sections (20um) makes it easier to handle the sample. Sections over 100um can be stained free floating then gently transferred to the coverslip with a kiddie paint brush.

Med vänlig hälsning / Best regards
Sylvie
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Sylvie Le Guyader, PhD
Live Cell Imaging Facility Manager
Karolinska Institutet- Bionut Dpt
Blickagången 16,
Room 7362 (lab)/7840 (office)
14157 Huddinge, Sweden
mobile: [phone]
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LCI microscopy blog

-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On Behalf Of Erika Wee
Sent: Tuesday, 23 June, 2020 20:04
To: [hidden email]
Subject: Re: Mounting tissue section onto the coverslip

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Dear Rhonda,

Thank you so much for the detailed info, that's super helpful. Sorry if my previous message wasn't clear, there were so many different kind of badly prepared sample I have seen people bringing in to try to image on the facility microscopes.

I realize it's too much to ask and too technical demanding microscopy beginners to mount tissue onto the coverslip, right now I advised them to practice to avoid bubbles as much as they can, but eventually one day if they would like to do higher resolution imaging, it's still critical to mount tissues onto the coverslips.

And yes, the tissues are a bit wrinkled as well, any suggestion would be greatly appreciated.

Thanks again.

Best,
Erika


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