Re: Mounting tissue section onto the coverslip

Posted by Martin Wessendorf-5 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Mounting-tissue-section-onto-the-coverslip-tp7591021p7591042.html

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Dear Erika--

It looks as if all of your responses have been emailed directly to you,
rather than to the list.  (Or to be more clear: I haven't seen any of the
responses!).  Would you mind sharing any protocols for making sections to
stick to coverslips?  I expect there are others out there who would
appreciate that--especially if you're making it work for RNA localization.

Thanks--

Martin Wessendorf
List Manager

On Tue, Jun 23, 2020 at 1:06 PM Erika Wee <[hidden email]> wrote:

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>
> Dear Rhonda,
>
> Thank you so much for the detailed info, that's super helpful. Sorry if my
> previous message wasn't clear, there were so many different kind of badly
> prepared sample I have seen people bringing in to try to image on the
> facility microscopes.
>
> I realize it's too much to ask and too technical demanding microscopy
> beginners to mount tissue onto the coverslip, right now I advised them to
> practice to avoid bubbles as much as they can, but eventually one day if
> they would like to do higher resolution imaging, it's still critical to
> mount tissues onto the coverslips.
>
> And yes, the tissues are a bit wrinkled as well, any suggestion would be
> greatly appreciated.
>
> Thanks again.
>
> Best,
> Erika
>