Re: Mounting tissue section onto the coverslip

Posted by Martin Wessendorf-5 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Mounting-tissue-section-onto-the-coverslip-tp7591021p7591044.html

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Dear All--

It looks as if at least some people received all of the responses to
Erika's post--also, the responses can be found on the web version of the
Confocal List.

Did anyone else NOT receive those responses?  You all should have received
several posts with the subject line: "Re: Mounting tissue section onto the
coverslip" with the sender NOT being Erika Wee.

Feel free to respond to me directly rather than cluttering the List with
this!

Thanks for your help trouble-shooting--

Martin Wessendorf

On Wed, Jun 24, 2020 at 9:15 AM Martin Wessendorf <[hidden email]> wrote:

> Dear Erika--
>
> It looks as if all of your responses have been emailed directly to you,
> rather than to the list.  (Or to be more clear: I haven't seen any of the
> responses!).  Would you mind sharing any protocols for making sections to
> stick to coverslips?  I expect there are others out there who would
> appreciate that--especially if you're making it work for RNA localization.
>
> Thanks--
>
> Martin Wessendorf
> List Manager
>
> On Tue, Jun 23, 2020 at 1:06 PM Erika Wee <[hidden email]> wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> Post images on http://www.imgur.com and include the link in your posting.
>> *****
>>
>> Dear Rhonda,
>>
>> Thank you so much for the detailed info, that's super helpful. Sorry if
>> my previous message wasn't clear, there were so many different kind of
>> badly prepared sample I have seen people bringing in to try to image on the
>> facility microscopes.
>>
>> I realize it's too much to ask and too technical demanding microscopy
>> beginners to mount tissue onto the coverslip, right now I advised them to
>> practice to avoid bubbles as much as they can, but eventually one day if
>> they would like to do higher resolution imaging, it's still critical to
>> mount tissues onto the coverslips.
>>
>> And yes, the tissues are a bit wrinkled as well, any suggestion would be
>> greatly appreciated.
>>
>> Thanks again.
>>
>> Best,
>> Erika
>>
>