Re: Aberration correction

Posted by PAVAK SHAH on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Aberration-correction-tp7591120p7591121.html

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi Antonio,

I have used Olympus objectives on a Zeiss body before (the 60x silicone oil
objective especially) and the mismatch causes chromatic aberration  that
results in a ~ 0.7 micron offset between ~green and ~red channels.
Depending on the application, this is straightforward to correct for using
multicolored beads and the SPIM registration plugin for Fiji by Stephan
Priebisch and may be worth the advantages of the particular objective you
want to use. Whether the degree of aberration will be similar on a Nikon
frame, I'm not sure but it should be easy to check.

Best,
Pavak

On Sat, Aug 1, 2020, 7:59 AM Antonio Jose Pereira <
[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear all,
>
> I will test an Olympus objective on a Nikon Ti ...
> I know that Olympus does all the chromatic aberration correction at the
> objective, but am unsure if Nikon distributes this correction between the
> objective and the tube lens. I would assume that the infinity path is
> already 'fully' corrected ... but would like to be sure. I appreciate any
> info on this.
>
> Best,
>
> Antonio Pereira
> CID lab
> i3S/IBMC, Universidade do Porto
> Room 001.S2B, [phone] Ext. 6127
>