Re: Aberration correction

Posted by George McNamara on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Aberration-correction-tp7591120p7591122.html

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Olympus, Nikon and Leica do all corrections in the objective lens.

Zeiss does some correction in tube lens.

George

p.s. in principle (maybe not in practice!) deconvolution software should
be able to correct aberrations.

p.p.s. deconvolution - Hari Shroff's lab and collaborators have a new
fast deconvolution paper, Guo et al 2020 Nature Biotechnology
https://www.nature.com/articles/s41587-020-0560-x with matlab/imagej
software and code in online supplement (text also mentions it posted to
github). Part of the abstract:

"Here we describe theoretical and practical advances in algorithm and
software design that result in image processing times that are tenfold
to several thousand fold faster than with previous methods. First, we
show that an ‘unmatched back projector’ accelerates deconvolution
relative to the classic Richardson–Lucy algorithm by at least tenfold.
Second, three-dimensional image-based registration with a graphics
processing unit enhances processing speed 10- to 100-fold over CPU
processing."

On 8/1/2020 10:58 AM, Antonio Jose Pereira wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear all,
>
> I will test an Olympus objective on a Nikon Ti ...
> I know that Olympus does all the chromatic aberration correction at the objective, but am unsure if Nikon distributes this correction between the objective and the tube lens. I would assume that the infinity path is already 'fully' corrected ... but would like to be sure. I appreciate any info on this.
>
> Best,
>
> Antonio Pereira
> CID lab
> i3S/IBMC, Universidade do Porto
> Room 001.S2B, [phone] Ext. 6127