Re: Aberration correction

Posted by Craig Brideau on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Aberration-correction-tp7591120p7591124.html

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I've heard that Leica does a bit of correction on the violet end in the
tube lens, but have not verified personally. Zeiss does quite a bit of
correction in its tube lens design so you should only use Zeiss with Zeiss.
I have interchanged Nikon and Olympus freely without major issue, although
since Olympus is designed for a 180mm tube lens and Nikon for 200mm, your
field and magnification will be slightly different than usual.

Craig

On Sat, Aug 1, 2020 at 9:59 AM George McNamara <[hidden email]>
wrote:

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> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Olympus, Nikon and Leica do all corrections in the objective lens.
>
> Zeiss does some correction in tube lens.
>
> George
>
> p.s. in principle (maybe not in practice!) deconvolution software should
> be able to correct aberrations.
>
> p.p.s. deconvolution - Hari Shroff's lab and collaborators have a new
> fast deconvolution paper, Guo et al 2020 Nature Biotechnology
> https://www.nature.com/articles/s41587-020-0560-x with matlab/imagej
> software and code in online supplement (text also mentions it posted to
> github). Part of the abstract:
>
> "Here we describe theoretical and practical advances in algorithm and
> software design that result in image processing times that are tenfold
> to several thousand fold faster than with previous methods. First, we
> show that an ‘unmatched back projector’ accelerates deconvolution
> relative to the classic Richardson–Lucy algorithm by at least tenfold.
> Second, three-dimensional image-based registration with a graphics
> processing unit enhances processing speed 10- to 100-fold over CPU
> processing."
>
> On 8/1/2020 10:58 AM, Antonio Jose Pereira wrote:
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > Post images on http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> > Dear all,
> >
> > I will test an Olympus objective on a Nikon Ti ...
> > I know that Olympus does all the chromatic aberration correction at the
> objective, but am unsure if Nikon distributes this correction between the
> objective and the tube lens. I would assume that the infinity path is
> already 'fully' corrected ... but would like to be sure. I appreciate any
> info on this.
> >
> > Best,
> >
> > Antonio Pereira
> > CID lab
> > i3S/IBMC, Universidade do Porto
> > Room 001.S2B, [phone] Ext. 6127
>