Re: Aberration correction

Posted by Antonio Jose Pereira-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Aberration-correction-tp7591120p7591126.html

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Great. Thank you all. The question is answered.

Now I wonder why some companies choose to split the correction between the objective and the tube lens. Unless the achieved performance gains from the splitting, we'll then have an infinity path segment which is sub-optimal for injection of e.g. ultra-short pulses. If I'm thinking correctly, the infinity segment is then only 'infinite' at some spectral points after all.

Thanks,
Antonio

----- Original Message -----
From: "Craig Brideau" <[hidden email]>
To: "CONFOCALMICROSCOPY" <[hidden email]>
Sent: Saturday, 1 August, 2020 18:56:35
Subject: Re: Aberration correction

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I've heard that Leica does a bit of correction on the violet end in the
tube lens, but have not verified personally. Zeiss does quite a bit of
correction in its tube lens design so you should only use Zeiss with Zeiss.
I have interchanged Nikon and Olympus freely without major issue, although
since Olympus is designed for a 180mm tube lens and Nikon for 200mm, your
field and magnification will be slightly different than usual.

Craig

On Sat, Aug 1, 2020 at 9:59 AM George McNamara <[hidden email]>
wrote:

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> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Olympus, Nikon and Leica do all corrections in the objective lens.
>
> Zeiss does some correction in tube lens.
>
> George
>
> p.s. in principle (maybe not in practice!) deconvolution software should
> be able to correct aberrations.
>
> p.p.s. deconvolution - Hari Shroff's lab and collaborators have a new
> fast deconvolution paper, Guo et al 2020 Nature Biotechnology
> https://www.nature.com/articles/s41587-020-0560-x with matlab/imagej
> software and code in online supplement (text also mentions it posted to
> github). Part of the abstract:
>
> "Here we describe theoretical and practical advances in algorithm and
> software design that result in image processing times that are tenfold
> to several thousand fold faster than with previous methods. First, we
> show that an ‘unmatched back projector’ accelerates deconvolution
> relative to the classic Richardson–Lucy algorithm by at least tenfold.
> Second, three-dimensional image-based registration with a graphics
> processing unit enhances processing speed 10- to 100-fold over CPU
> processing."
>
> On 8/1/2020 10:58 AM, Antonio Jose Pereira wrote:
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > Post images on http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> > Dear all,
> >
> > I will test an Olympus objective on a Nikon Ti ...
> > I know that Olympus does all the chromatic aberration correction at the
> objective, but am unsure if Nikon distributes this correction between the
> objective and the tube lens. I would assume that the infinity path is
> already 'fully' corrected ... but would like to be sure. I appreciate any
> info on this.
> >
> > Best,
> >
> > Antonio Pereira
> > CID lab
> > i3S/IBMC, Universidade do Porto
> > Room 001.S2B, [phone] Ext. 6127
>