Re: Aberration correction

Posted by Marc Reinig-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Aberration-correction-tp7591120p7591128.html

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You can pre-compensate the laser beam to counter the characteristics of the
lens/system.

Marco

On Sat, Aug 1, 2020 at 12:59 Antonio Jose Pereira <
[hidden email]> wrote:

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>
> Great. Thank you all. The question is answered.
>
> Now I wonder why some companies choose to split the correction between the
> objective and the tube lens. Unless the achieved performance gains from the
> splitting, we'll then have an infinity path segment which is sub-optimal
> for injection of e.g. ultra-short pulses. If I'm thinking correctly, the
> infinity segment is then only 'infinite' at some spectral points after all.
>
> Thanks,
> Antonio
>
> ----- Original Message -----
> From: "Craig Brideau" <[hidden email]>
> To: "CONFOCALMICROSCOPY" <[hidden email]>
> Sent: Saturday, 1 August, 2020 18:56:35
> Subject: Re: Aberration correction
>
> *****
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> *****
>
> I've heard that Leica does a bit of correction on the violet end in the
> tube lens, but have not verified personally. Zeiss does quite a bit of
> correction in its tube lens design so you should only use Zeiss with Zeiss.
> I have interchanged Nikon and Olympus freely without major issue, although
> since Olympus is designed for a 180mm tube lens and Nikon for 200mm, your
> field and magnification will be slightly different than usual.
>
> Craig
>
> On Sat, Aug 1, 2020 at 9:59 AM George McNamara <[hidden email]>
> wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > Post images on http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> > Olympus, Nikon and Leica do all corrections in the objective lens.
> >
> > Zeiss does some correction in tube lens.
> >
> > George
> >
> > p.s. in principle (maybe not in practice!) deconvolution software should
> > be able to correct aberrations.
> >
> > p.p.s. deconvolution - Hari Shroff's lab and collaborators have a new
> > fast deconvolution paper, Guo et al 2020 Nature Biotechnology
> > https://www.nature.com/articles/s41587-020-0560-x with matlab/imagej
> > software and code in online supplement (text also mentions it posted to
> > github). Part of the abstract:
> >
> > "Here we describe theoretical and practical advances in algorithm and
> > software design that result in image processing times that are tenfold
> > to several thousand fold faster than with previous methods. First, we
> > show that an ‘unmatched back projector’ accelerates deconvolution
> > relative to the classic Richardson–Lucy algorithm by at least tenfold.
> > Second, three-dimensional image-based registration with a graphics
> > processing unit enhances processing speed 10- to 100-fold over CPU
> > processing."
> >
> > On 8/1/2020 10:58 AM, Antonio Jose Pereira wrote:
> > > *****
> > > To join, leave or search the confocal microscopy listserv, go to:
> > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > > Post images on http://www.imgur.com and include the link in your
> > posting.
> > > *****
> > >
> > > Dear all,
> > >
> > > I will test an Olympus objective on a Nikon Ti ...
> > > I know that Olympus does all the chromatic aberration correction at the
> > objective, but am unsure if Nikon distributes this correction between the
> > objective and the tube lens. I would assume that the infinity path is
> > already 'fully' corrected ... but would like to be sure. I appreciate any
> > info on this.
> > >
> > > Best,
> > >
> > > Antonio Pereira
> > > CID lab
> > > i3S/IBMC, Universidade do Porto
> > > Room 001.S2B, +351 22 607 49 59 Ext. 6127
> >
>
--
Marco

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