Re: Size of a bleach point

Posted by pkshah on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Size-of-a-bleach-point-tp7590808p7591144.html

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Hi Xavi,

Are you trying to estimate the size of the region that is experiencing a
temperature change? Many fluorescent proteins are actually reversibly
temperature sensitive in their brightness and a number of folks have shown
that it's possible to measure the temperature achieved at a laser focus by
comparing against a calibration curve of FP brightness vs temperature. See:

https://www.nature.com/articles/ncomms14100

https://www.nature.com/articles/s41592-018-0168-y?WT.feed_name=subjects_experimental-organisms

This may not work if you're heating to the point of bleaching, however.

Best,
Pavak


On Fri, Apr 24, 2020, 9:56 AM SANJUAN SAMARRA, XAVIER <
[hidden email]> wrote:

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> Post images on http://www.imgur.com and include the link in your posting.
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>
> Thanks for all the feedback! I did not want this for a photobleaching
> experiment, we have been using a multiphoton laser at 830 nm to heat
> nanoparticles within cells, but I guess this does not affect the
> calculations!
>
> Best,
>
> Xavi.
>
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> *Xavier Sanjuan*
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