Posted by
Rebecca M. Williams on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Zeiss-880-timing-question-tp7591169p7591171.html
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Hi Michael --
As I recall this info is remarkably difficult to get. The only way I could get it was to have zen display a timepoint on the images. You do this using the graphics tab under the display. Make sure you have "show all" checked (hidden to the right). Choose the More. . . option and then coordinate -> time.
Becky
Rebecca Williams
Director, BRC Imaging Facility
B35 Weill Hall
Cornell University
(607) 255-4610
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Sent: Wednesday, August 19, 2020 4:44 PM
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Subject: Zeiss 880 timing question
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We have been trying to do FRAP experiments on the Zeiss 880, but we are having difficulties getting accurate times.
Typically, we take 2 to 4 before images, then a bleach, and then the recovery sequence.
The problem is that we do not know how to get accurate times for the sequence after the bleaching. I used to do this on a Leica SP2 and the times were well reported in the metadata, but I cannot find the times in the Zeiss files. Any help would be appreciated.
Thank you.
Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory
NYU Langone Health, 540 First Avenue, SK2 Microscopy Suite, New York, NY 10016
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