Re: Zeiss 880 timing question

Posted by Cammer, Michael-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Zeiss-880-timing-question-tp7591169p7591174.html

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I want to thank the many replies to my question including from Zeiss directly.

I had been struggling with the regular metadata and didn't even think to look at the XML version separately.  

This may also answer a question someone asked about FRAP a few weeks ago.  In addition to the Elyra images, I opened a FRAP sequence from the Zeiss 880 and while not all the information to reconstruct the settings is displayed, this combined with the ROIs may be sufficient to run the analyses.

Best regards-

Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory
NYU Langone Health, 540 First Avenue, SK2 Microscopy Suite, New York, NY  10016
Office: 646-501-0567 Cell: 914-309-3270  [hidden email]  
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-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On Behalf Of Toth, Peter Tibor
Sent: Thursday, August 20, 2020 12:35 PM
To: [hidden email]
Subject: Re: Zeiss 880 timing question

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Hi Michael,

You can drop the Zeiss .czi file into Fiji. In the Metadata viewing column check the "Display OME-XML metadata" checkbox.  Metadata with times, etc.  opens up in a separate window.

-Peter






--

Peter T. Toth, PhD

Director, Fluorescence Imaging Core

The University of Illinois at Chicago

835 S. Wolcott Ave (MSB), E-325

Phone: [phone]

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From: Confocal Microscopy List <[hidden email]> on behalf of Cammer, Michael <[hidden email]>
Sent: Wednesday, August 19, 2020 3:43 PM
To: [hidden email] <[hidden email]>
Subject: Zeiss 880 timing question

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We have been trying to do FRAP experiments on the Zeiss 880, but we are having difficulties getting accurate times.


Typically, we take 2 to 4 before images, then a bleach, and then the recovery sequence.


The problem is that we do not know how to get accurate times for the sequence after the bleaching.  I used to do this on a Leica SP2 and the times were well reported in the metadata, but I cannot find the times in the Zeiss files.  Any help would be appreciated.


Thank you.


Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory

NYU Langone Health, 540 First Avenue, SK2 Microscopy Suite, New York, NY  10016

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