Re: Numerical aperture and spatial resolution

Posted by Davide Accardi-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Numerical-aperture-and-spatial-resolution-tp7591293p7591297.html

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Hi Javier,

This is a video with Peter Evennett, senior microscopist of the RMS, that explains it all.
Peter reproduces Abbe’s experiments and clearly demonstrates how resolution depends on lambda and NA.

https://youtu.be/60_jgZtyR6U

Good luck!

Davide
--
Davide Accardi, PhD.
Champalimaud ABBE Platform
Advanced BioImaging and BioOptics Experimental Platform
Scientific and Managing Leader

Champalimaud Centre for the Unknown
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>> On 19. Sep 2020, at 10:40, F Javier Diez Guerra <[hidden email]> wrote:
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> To join, leave or search the confocal microscopy listserv, go to:
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> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hello,
>
> I wonder if anybody in the list could help.
>
> I want to convey to biology undergraduate students (very allergic to physics and mathematics) the understanding of the relationship between numerical aperture and spatial resolution.
>
> I have already given them links to the different microscopy primer sites. They find difficult to understand why the airy disk is generated in the image plane, how the diffraction orders affect resolution and why increasing NA reduces the image spot.
>
> Could anybody share a basic and intuitive infographic slide, animation or any other resource that could help to ease comprehension in this context?
>
> Thanks
>
> Javier
>
>
> --
> Fco. Javier Diez-Guerra, PhD
>
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