http://confocal-microscopy-list.275.s1.nabble.com/Numerical-aperture-and-spatial-resolution-tp7591293p7591299.html
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> Hi Javier,
>
> I suggest you ask the class if they have any interest in taking photos
> with their smartphone or DSLR camera, and if they do, bring in a DSLR
> and tripod and computer/projector (if the room does not have that
> already), explain f/stop and NA are inverse of each other, and
> demonstrate NA (resolution, intensity, depth of field) on the class by
> focusing on faces in the middle row. Wavelength: yes, you could buy some
> filters for the camera lens (or deal with separating channels in
> Photoshop, fiji ImageJ, etc), but probably better done on a microscope.
>
> George
>
> p.s. this post was inspired by a conversation yesterday -- Friday happy
> hour -- with a colleague, Prof. Jim Potter, who told me about a
> conversation he had with someone with a very expensive digital camera
> hobby, who only used their cameras set to auto. Jim explained f/stop,
> ISO and more (framing scene etc). My thanks to Jim for good story with
> nice timing and especially foraging for the beverages.
>
> A fun (in theory, but probably not practical or wise) wavelength test
> would be to use bring in blue (~400 nm) and NIR (~800nm would be nice,
> values chosen to be 2 fold, not necessarily practical) and a smoke
> machine (and some 400nm and NIR friendly lighting in case room lights
> too dim at those wavelength), and demonstration resolution vs ability to
> see through the smoke (Mie scattering etc). If you do this, and the
> smoke alarm turns on, your school may not be happy with you (though all
> the students may be happy to escape class early).
>
> Of course if they vote no to learning about f/stop and NA, you can ask
> them how they expect to get to a million TikTok followers each without
> knowing how to take good quality videos. Come ot think of it, if they do
> vote yes, and you put your demo on your TikTok feed and make a lot of
> money, you can send me the URL and a thank you check.
>
> On 9/19/2020 5:38 AM, F Javier Diez Guerra wrote:
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> > To join, leave or search the confocal microscopy listserv, go to:
> >
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http://www.imgur.com and include the link in your
> posting.
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> >
> > Hello,
> >
> > I wonder if anybody in the list could help.
> >
> > I want to convey to biology undergraduate students (very allergic to
> > physics and mathematics) the understanding of the relationship between
> > numerical aperture and spatial resolution.
> >
> > I have already given them links to the different microscopy primer
> > sites. They find difficult to understand why the airy disk is
> > generated in the image plane, how the diffraction orders affect
> > resolution and why increasing NA reduces the image spot.
> >
> > Could anybody share a basic and intuitive infographic slide, animation
> > or any other resource that could help to ease comprehension in this
> > context?
> >
> > Thanks
> >
> > Javier
> >
> >
>