Re: [External] Re: Numerical aperture and spatial resolution

Posted by JOEL B. SHEFFIELD on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Numerical-aperture-and-spatial-resolution-tp7591293p7591303.html

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I agree.  This is excellent!  His analysis of phase contrast is about the
best I've seen.


Joel B. Sheffield, Ph.D
Department of Biology
Temple University
Philadelphia, PA 19122
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On Sun, Sep 20, 2020 at 8:42 AM Julio MATEOS_LANGERAK <
[hidden email]> wrote:

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>
> Hi,
>
> This is the same demonstration by Peter Evennett as a series of videos.
> The format is possibly more suited for teaching. It's one of the most
> beautyful demonstrations I've ever seen.
>
>
> https://www.youtube.com/watch?v=6mGEGs7vI00&list=PLN3i-H51ZELg6SZqAUIU3EI3F0_2igL_x&index=1
>
> Best, Julio
>
> ________________________________
> From: Confocal Microscopy List <[hidden email]> on
> behalf of Davide Accardi <[hidden email]>
> Sent: 19 September 2020 17:54
> To: [hidden email] <[hidden email]>
> Subject: Re: Numerical aperture and spatial resolution
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
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>
> Hi Javier,
>
> This is a video with Peter Evennett, senior microscopist of the RMS, that
> explains it all.
> Peter reproduces Abbe’s experiments and clearly demonstrates how
> resolution depends on lambda and NA.
>
> https://youtu.be/60_jgZtyR6U
>
> Good luck!
>
> Davide
> --
> Davide Accardi, PhD.
> Champalimaud ABBE Platform
> Advanced BioImaging and BioOptics Experimental Platform
> Scientific and Managing Leader
>
> Champalimaud Centre for the Unknown
> Av. Brasilia, Doca de Pedroucos
> 1400-038 Lisbon, Portugal
> T ([phone]
>
> www.fchampalimaud.org<http://www.fchampalimaud.org>
> [hidden email]
>
> Sent from my iPhone
>
> >> On 19. Sep 2020, at 10:40, F Javier Diez Guerra <[hidden email]>
> wrote:
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
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> posting.
> > *****
> >
> > Hello,
> >
> > I wonder if anybody in the list could help.
> >
> > I want to convey to biology undergraduate students (very allergic to
> physics and mathematics) the understanding of the relationship between
> numerical aperture and spatial resolution.
> >
> > I have already given them links to the different microscopy primer
> sites. They find difficult to understand why the airy disk is generated in
> the image plane, how the diffraction orders affect resolution and why
> increasing NA reduces the image spot.
> >
> > Could anybody share a basic and intuitive infographic slide, animation
> or any other resource that could help to ease comprehension in this context?
> >
> > Thanks
> >
> > Javier
> >
> >
> > --
> > Fco. Javier Diez-Guerra, PhD
> >
> > Servicio de Microscopía Confocal
> > Centro de Biologia Molecular Severo Ochoa
> > C/ Nicolás Cabrera, 1
> > Campus de Cantoblanco
> > 28049 Madrid
> > SPAIN
> >
> > Tel     [phone]
> > e-mail: [hidden email]
>