Re: Protective window for a confocal microscope?

Posted by Avi Jacob on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Protective-window-for-a-confocal-microscope-tp7591445p7591451.html

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Maybe I misunderstand the issue, but if it's because of oil leaking off oil
objectives and getting into the insides, I use these ponytail elastic
fluffy things on every oil objective. (I don't understand why Doug does not
like em. Doug, maybe you did not use ones that can soak up oil well
enough?).
Just stretch one a bit, and place it on the objective. It will soak up any
oil and when saturated, just throw it out.  Every month or so I switch them
out and I sleep soundly - I have zero oil spilling off the objectives. The
amazon link is just to see specifically what I mean, you can pick these up
all over (took me some minutes to find that exact type). I luckily found a
package with perfectly sized ones (in my daughter's drawer).

https://www.amazon.com/Elastic-Bands-Rubber-Ponytail-Holders/dp/B075FLPVYT
--
Avi Jacob, Ph.D.
Head of The Kanbar Light Microscopy Unit
The Goodman Faculty of Life Sciences
Bar-Ilan University, Ramat-Gan 5290002, Israel




On Tue, Oct 27, 2020 at 1:45 PM Doug Richardson <[hidden email]>
wrote:

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>
> I would highly recommend the aquastop system from Zeiss. It is overpriced
> for some pieces of plastic, but it forms a very tight seal and pays for
> itself many times over. It is far superior to hair elastics/glove
> fingers/condoms which we used in the past.
>
> Doug
>
> Get Outlook for Android
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> ________________________________
> From: Confocal Microscopy List <[hidden email]> on
> behalf of Tomek Węgierski <[hidden email]>
> Sent: Tuesday, October 27, 2020 7:12:10 AM
> To: [hidden email] <[hidden email]>
> Subject: Protective window for a confocal microscope?
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
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>
> Dear All,
> I am considering a purchase of a protective window for our Zeiss LSM800
> and I would like to hear your advice whether this is a good idea, and if
> yes, which one to buy.
> The reason is our LSM800 is on an inverted stand and we already got a
> leakage of oil once which went down onto Optovar. And this was despite my
> regular checks of objectives (both sides) for leaking oil and extensive
> education of users on how important it is not to use too much oil and to
> clean lenses, especially on inverted stands.
> Zeiss Observer has a dummy slider with an opening for 32 mm filters. I
> hope, a protective window there should stop the oil, if the leakage happens
> again.
> 1) do you think placing a protective window in a confocal microscope is a
> good idea or not really, because the imaging will be affected? This would
> be in infinity space, so I guess it should not be detrimental....
> 2) which characteristics of protective window I should take into
> consideration (substrate, thickness, coating, flatness, surface quality,
> parallelism)? Currently I am inclined into protective glasses by Edmund
> Optics such as 1-mm thick MgF2-coated Sapphire window, which has high
> transmission, or 2-mm thick MgF2-coated B270 window, which has a bit worse
> transmission but better surface parameters (and is much cheaper).
> best regards,
> Tomasz
>
>
> Tomasz Wegierski, PhD
> International Institute of Molecular and Cell Biology
> Trojdena 4, 02-109 Warsaw, POLAND
> tel: [phone]
> fax: [phone]
> http://www.iimcb.gov.pl/
>