Re: Protective window for a confocal microscope?

Posted by Cammer, Michael-2 on Oct 27, 2020; 1:12pm
URL: http://confocal-microscopy-list.275.s1.nabble.com/Protective-window-for-a-confocal-microscope-tp7591445p7591453.html

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Aquastop works well for big spills when installed correctly and has proven effective on some of our Zeiss scopes.  But we did have a user who spilled over or around it on our Zeiss 710, so it is not foolproof.


Hair elastics are great for the oil.


Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory

NYU Langone Health, 540 First Avenue, SK2 Microscopy Suite, New York, NY  10016

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From: Confocal Microscopy List <[hidden email]> on behalf of Doug Richardson <[hidden email]>
Sent: Tuesday, October 27, 2020 7:41:45 AM
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Subject: Re: Protective window for a confocal microscope?

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I would highly recommend the aquastop system from Zeiss. It is overpriced for some pieces of plastic, but it forms a very tight seal and pays for itself many times over. It is far superior to hair elastics/glove fingers/condoms which we used in the past.

Doug

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From: Confocal Microscopy List <[hidden email]> on behalf of Tomek Węgierski <[hidden email]>
Sent: Tuesday, October 27, 2020 7:12:10 AM
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Subject: Protective window for a confocal microscope?

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Dear All,
I am considering a purchase of a protective window for our Zeiss LSM800 and I would like to hear your advice whether this is a good idea, and if yes, which one to buy.
The reason is our LSM800 is on an inverted stand and we already got a leakage of oil once which went down onto Optovar. And this was despite my regular checks of objectives (both sides) for leaking oil and extensive education of users on how important it is not to use too much oil and to clean lenses, especially on inverted stands.
Zeiss Observer has a dummy slider with an opening for 32 mm filters. I hope, a protective window there should stop the oil, if the leakage happens again.
1) do you think placing a protective window in a confocal microscope is a good idea or not really, because the imaging will be affected? This would be in infinity space, so I guess it should not be detrimental....
2) which characteristics of protective window I should take into consideration (substrate, thickness, coating, flatness, surface quality, parallelism)? Currently I am inclined into protective glasses by Edmund Optics such as 1-mm thick MgF2-coated Sapphire window, which has high transmission, or 2-mm thick MgF2-coated B270 window, which has a bit worse transmission but better surface parameters (and is much cheaper).
best regards,
Tomasz


Tomasz Wegierski, PhD
International Institute of Molecular and Cell Biology
Trojdena 4, 02-109 Warsaw, POLAND
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