Re: Protective window for a confocal microscope?

Posted by Lemasters, John J. on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Protective-window-for-a-confocal-microscope-tp7591445p7591454.html

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Women in the US at least will recognize "ponytail elastic fluffy things" as "scrunchies", which you can buy under this name from Amazon and other suppliers. We use them also. They are helpful in a multi-user environment where inexperienced users can get overly exuberant in oiling objectives. They should be replaced when oil drips on them.

Best, John

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John J. Lemasters, MD, PhD
Professor and GlaxoSmithKline Distinguished Endowed Chair
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Medical University of South Carolina
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-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On Behalf Of Avi Jacob
Sent: Tuesday, October 27, 2020 9:06 AM
To: [hidden email]
Subject: Re: Protective window for a confocal microscope?

CAUTION: External

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Maybe I misunderstand the issue, but if it's because of oil leaking off oil objectives and getting into the insides, I use these ponytail elastic fluffy things on every oil objective. (I don't understand why Doug does not like em. Doug, maybe you did not use ones that can soak up oil well enough?).
Just stretch one a bit, and place it on the objective. It will soak up any oil and when saturated, just throw it out.  Every month or so I switch them out and I sleep soundly - I have zero oil spilling off the objectives. The amazon link is just to see specifically what I mean, you can pick these up all over (took me some minutes to find that exact type). I luckily found a package with perfectly sized ones (in my daughter's drawer).

https://urldefense.com/v3/__https://www.amazon.com/Elastic-Bands-Rubber-Ponytail-Holders/dp/B075FLPVYT__;!!Ab1_Rw!Skra7YcxZVCzHTYZgYmYyd8oaFrWhQjgg2qsfZlzOaztVPV5nx3K6ZEjzY16r31RiXc$
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Avi Jacob, Ph.D.
Head of The Kanbar Light Microscopy Unit The Goodman Faculty of Life Sciences Bar-Ilan University, Ramat-Gan 5290002, Israel




On Tue, Oct 27, 2020 at 1:45 PM Doug Richardson <[hidden email]>
wrote:

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> I would highly recommend the aquastop system from Zeiss. It is
> overpriced for some pieces of plastic, but it forms a very tight seal
> and pays for itself many times over. It is far superior to hair
> elastics/glove fingers/condoms which we used in the past.
>
> Doug
>
> Get Outlook for Android
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> behalf of Tomek Węgierski <[hidden email]>
> Sent: Tuesday, October 27, 2020 7:12:10 AM
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> Dear All,
> I am considering a purchase of a protective window for our Zeiss
> LSM800 and I would like to hear your advice whether this is a good
> idea, and if yes, which one to buy.
> The reason is our LSM800 is on an inverted stand and we already got a
> leakage of oil once which went down onto Optovar. And this was despite
> my regular checks of objectives (both sides) for leaking oil and
> extensive education of users on how important it is not to use too
> much oil and to clean lenses, especially on inverted stands.
> Zeiss Observer has a dummy slider with an opening for 32 mm filters. I
> hope, a protective window there should stop the oil, if the leakage
> happens again.
> 1) do you think placing a protective window in a confocal microscope
> is a good idea or not really, because the imaging will be affected?
> This would be in infinity space, so I guess it should not be detrimental....
> 2) which characteristics of protective window I should take into
> consideration (substrate, thickness, coating, flatness, surface
> quality, parallelism)? Currently I am inclined into protective glasses
> by Edmund Optics such as 1-mm thick MgF2-coated Sapphire window, which
> has high transmission, or 2-mm thick MgF2-coated B270 window, which
> has a bit worse transmission but better surface parameters (and is much cheaper).
> best regards,
> Tomasz
>
>
> Tomasz Wegierski, PhD
> International Institute of Molecular and Cell Biology Trojdena 4,
> 02-109 Warsaw, POLAND
> tel: [phone]
> fax: [phone]
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>