http://confocal-microscopy-list.275.s1.nabble.com/Protective-window-for-a-confocal-microscope-tp7591445p7591455.html
it's case upside down, and put it in a 37C oven. Ridiculous, in a bad way,
how much oil comes out. The 40X also doesn't have "lip" that acts as a bit
the stand). Takes me 15 minutes. Although I shouldn't have to do that ...
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>
> Aquastop works well for big spills when installed correctly and has proven
> effective on some of our Zeiss scopes. But we did have a user who spilled
> over or around it on our Zeiss 710, so it is not foolproof.
>
>
> Hair elastics are great for the oil.
>
>
> Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory
>
> NYU Langone Health, 540 First Avenue, SK2 Microscopy Suite, New York, NY
> 10016
>
>
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>
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> ________________________________
> From: Confocal Microscopy List <
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> behalf of Doug Richardson <
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> Sent: Tuesday, October 27, 2020 7:41:45 AM
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> Subject: Re: Protective window for a confocal microscope?
>
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>
> I would highly recommend the aquastop system from Zeiss. It is overpriced
> for some pieces of plastic, but it forms a very tight seal and pays for
> itself many times over. It is far superior to hair elastics/glove
> fingers/condoms which we used in the past.
>
> Doug
>
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>
> Dear All,
> I am considering a purchase of a protective window for our Zeiss LSM800
> and I would like to hear your advice whether this is a good idea, and if
> yes, which one to buy.
> The reason is our LSM800 is on an inverted stand and we already got a
> leakage of oil once which went down onto Optovar. And this was despite my
> regular checks of objectives (both sides) for leaking oil and extensive
> education of users on how important it is not to use too much oil and to
> clean lenses, especially on inverted stands.
> Zeiss Observer has a dummy slider with an opening for 32 mm filters. I
> hope, a protective window there should stop the oil, if the leakage happens
> again.
> 1) do you think placing a protective window in a confocal microscope is a
> good idea or not really, because the imaging will be affected? This would
> be in infinity space, so I guess it should not be detrimental....
> 2) which characteristics of protective window I should take into
> consideration (substrate, thickness, coating, flatness, surface quality,
> parallelism)? Currently I am inclined into protective glasses by Edmund
> Optics such as 1-mm thick MgF2-coated Sapphire window, which has high
> transmission, or 2-mm thick MgF2-coated B270 window, which has a bit worse
> transmission but better surface parameters (and is much cheaper).
> best regards,
> Tomasz
>
>
> Tomasz Wegierski, PhD
> International Institute of Molecular and Cell Biology
> Trojdena 4, 02-109 Warsaw, POLAND
> tel: +48-22 597 0763
> fax: +48 22 597 0715
>
>
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