Posted by
Michael Stanley on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Protective-window-for-a-confocal-microscope-tp7591445p7591456.html
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in the 'old days' in the core we used to cut filter paper and then bore a
hole with a cork-bore slightly smaller than the lens. slide 2-3 (or as many
as will fit) layers of the filter paper over the objective. this will not
stop a "flood" of oil (which sounds very possible for some) but it will hold
much more than you would think. and, the most important feature of this
little trick is that the oil will show very quickly on the paper and
hopefully will trigger a response.!
some of the darker filter papers will show the oil better, but this will
vary by manufacturer.
no commercial interest here, just trying to protect all the optics in the
system.
michael
c. michael stanley, phd
senior applications scientist
chroma technology corp.
10 imtec lane
bellows falls, vt 05101
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-----Original Message-----
From: Confocal Microscopy List <
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Of Gary Laevsky
Sent: Tuesday, October 27, 2020 10:19 AM
To:
[hidden email]
Subject: Re: Protective window for a confocal microscope?
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The hair ties work great with objectives that have enough of a neck for the
tie to sit on, next to the barrel.
Our 40X objective (most frequently used) has no/very small neck, so the hair
ties don't work.
I fairly regularly (bi-weekly) have to take the objective off, store it in
it's case upside down, and put it in a 37C oven. Ridiculous, in a bad way,
how much oil comes out. The 40X also doesn't have "lip" that acts as a bit
of a reservoir (unlike the 60 and 100).
On one of my heavily used systems, I have to go so far as to dismantle the
top (stage, objective turret, and fluorescent turret) to get to the piece of
glass that prevents leakage into the body (amazingly awesome feature of the
stand). Takes me 15 minutes. Although I shouldn't have to do that ...
On Tue, Oct 27, 2020 at 9:13 AM Cammer, Michael <
[hidden email]> wrote:
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> Aquastop works well for big spills when installed correctly and has
> proven effective on some of our Zeiss scopes. But we did have a user
> who spilled over or around it on our Zeiss 710, so it is not foolproof.
>
>
> Hair elastics are great for the oil.
>
>
> Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory
>
> NYU Langone Health, 540 First Avenue, SK2 Microscopy Suite, New York,
> NY
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> From: Confocal Microscopy List <
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> Sent: Tuesday, October 27, 2020 7:41:45 AM
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> Subject: Re: Protective window for a confocal microscope?
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> I would highly recommend the aquastop system from Zeiss. It is
> overpriced for some pieces of plastic, but it forms a very tight seal
> and pays for itself many times over. It is far superior to hair
> elastics/glove fingers/condoms which we used in the past.
>
> Doug
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> Dear All,
> I am considering a purchase of a protective window for our Zeiss
> LSM800 and I would like to hear your advice whether this is a good
> idea, and if yes, which one to buy.
> The reason is our LSM800 is on an inverted stand and we already got a
> leakage of oil once which went down onto Optovar. And this was despite
> my regular checks of objectives (both sides) for leaking oil and
> extensive education of users on how important it is not to use too
> much oil and to clean lenses, especially on inverted stands.
> Zeiss Observer has a dummy slider with an opening for 32 mm filters. I
> hope, a protective window there should stop the oil, if the leakage
> happens again.
> 1) do you think placing a protective window in a confocal microscope
> is a good idea or not really, because the imaging will be affected?
> This would be in infinity space, so I guess it should not be
> detrimental....
> 2) which characteristics of protective window I should take into
> consideration (substrate, thickness, coating, flatness, surface
> quality, parallelism)? Currently I am inclined into protective glasses
> by Edmund Optics such as 1-mm thick MgF2-coated Sapphire window, which
> has high transmission, or 2-mm thick MgF2-coated B270 window, which
> has a bit worse transmission but better surface parameters (and is much
> cheaper).
> best regards,
> Tomasz
>
>
> Tomasz Wegierski, PhD
> International Institute of Molecular and Cell Biology Trojdena 4,
> 02-109 Warsaw, POLAND
> tel: +48-22 597 0763
> fax: +48 22 597 0715
>
>
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--
Best,
Gary Laevsky, Ph.D.
Director, Confocal Imaging Facility
Nikon Center of Excellence
Co-Founder, North Atlantic Microscopy Society (NAMS)
https://namsmicroscopy.com/ Dept. of Molecular Biology Washington Rd.
Princeton University
Princeton, New Jersey, 08544-1014
(O) 609 258 5432
(C) 508 507 1310