http://confocal-microscopy-list.275.s1.nabble.com/Protective-window-for-a-confocal-microscope-tp7591445p7591457.html
Scrunchies work well but are not fool-proof. Cotton not polyester material are more absorbent.
In addition to scrunchies I suggest wrapping the lens barrel near the nose of the objective with plumbers tape to prevent oil from seeping into the barrel. It can be done so that the lens retraction still functions. Then once the lens is on the nosepiece of the scope the plumbers tape can be wrapped there as well.
I've also used thin rubber sheets or parafilm sheets, as well as filter paper (thanks Mike S.!), to draw oil away from the lens and nosepiece.
Best method, however, is to teach users proper oil application and ensure they clean up after themselves.
St. Jude Children’s Research Hospital
“Images were acquired at the Cell & Tissue Imaging Center which is supported by SJCRH and NCI P30 CA021765.”
in the 'old days' in the core we used to cut filter paper and then bore a hole with a cork-bore slightly smaller than the lens. slide 2-3 (or as many as will fit) layers of the filter paper over the objective. this will not stop a "flood" of oil (which sounds very possible for some) but it will hold much more than you would think. and, the most important feature of this little trick is that the oil will show very quickly on the paper and hopefully will trigger a response.!
some of the darker filter papers will show the oil better, but this will vary by manufacturer.
no commercial interest here, just trying to protect all the optics in the system.
c. michael stanley, phd
The hair ties work great with objectives that have enough of a neck for the tie to sit on, next to the barrel.
Our 40X objective (most frequently used) has no/very small neck, so the hair ties don't work.
I fairly regularly (bi-weekly) have to take the objective off, store it in it's case upside down, and put it in a 37C oven. Ridiculous, in a bad way, how much oil comes out. The 40X also doesn't have "lip" that acts as a bit of a reservoir (unlike the 60 and 100).
On one of my heavily used systems, I have to go so far as to dismantle the top (stage, objective turret, and fluorescent turret) to get to the piece of glass that prevents leakage into the body (amazingly awesome feature of the stand). Takes me 15 minutes. Although I shouldn't have to do that ...
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> Aquastop works well for big spills when installed correctly and has
> proven effective on some of our Zeiss scopes. But we did have a user
> who spilled over or around it on our Zeiss 710, so it is not foolproof.
>
>
> Hair elastics are great for the oil.
>
>
> Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory
>
> NYU Langone Health, 540 First Avenue, SK2 Microscopy Suite, New York,
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> I would highly recommend the aquastop system from Zeiss. It is
> overpriced for some pieces of plastic, but it forms a very tight seal
> and pays for itself many times over. It is far superior to hair
> elastics/glove fingers/condoms which we used in the past.
>
> Doug
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> Dear All,
> I am considering a purchase of a protective window for our Zeiss
> LSM800 and I would like to hear your advice whether this is a good
> idea, and if yes, which one to buy.
> The reason is our LSM800 is on an inverted stand and we already got a
> leakage of oil once which went down onto Optovar. And this was despite
> my regular checks of objectives (both sides) for leaking oil and
> extensive education of users on how important it is not to use too
> much oil and to clean lenses, especially on inverted stands.
> Zeiss Observer has a dummy slider with an opening for 32 mm filters. I
> hope, a protective window there should stop the oil, if the leakage
> happens again.
> 1) do you think placing a protective window in a confocal microscope
> is a good idea or not really, because the imaging will be affected?
> This would be in infinity space, so I guess it should not be
> detrimental....
> 2) which characteristics of protective window I should take into
> consideration (substrate, thickness, coating, flatness, surface
> quality, parallelism)? Currently I am inclined into protective glasses
> by Edmund Optics such as 1-mm thick MgF2-coated Sapphire window, which
> has high transmission, or 2-mm thick MgF2-coated B270 window, which
> has a bit worse transmission but better surface parameters (and is
> much cheaper).
> best regards,
> Tomasz
>
>
> Tomasz Wegierski, PhD
> International Institute of Molecular and Cell Biology Trojdena 4,
> 02-109 Warsaw, POLAND
> tel: +48-22 597 0763
> fax: +48 22 597 0715
>
>
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