http://confocal-microscopy-list.275.s1.nabble.com/Protective-window-for-a-confocal-microscope-tp7591445p7591462.html
back to your question. The filter holder is there to hold a filter. Any
bet. Hard coating is a must, if you want to wipe oil off of it regularly.
Sapphire is hard to work with and comes with a price premium. Fused silica,
and look at some beads. If you're not able to tell the difference, you're
good to go. You can even try a glass coverslip, the 4% loss (the excitation
noticeable. Coverslips are not polished, so the TWE is generally bad, but
> *****
> To join, leave or search the confocal microscopy listserv, go to:
>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> Post images on
http://www.imgur.com and include the link in your posting.
> *****
>
> Dear All,
>
> Thank you very much for so many responses, although the discussion went a
> bit into a direction that I did not anticipate ??
> But yes, it is all about preventing oil from getting into the microscope.
> Our 40x/1.3 Fluar lens takes a lot of oil and I frequently have to clean
> its bottom side from oil. In contrast, I never saw any oil on the back of
> 63x/1.4 even though it is used as frequently as the 40x.
>
> I have to admit I have been skeptical about hair elastics, because when
> they saturate with oil they will just let it go. IMHO, it is replacing "oil
> cleaning" approach with "oil buffering" approach. The "cleaning" approach
> has worked for us for many years. It failed when an unexperienced user (who
> apparently did not take seriously what I was talking during the training)
> made two mistakes: did not clean the lens and let it stay in the optic path
> after the work instead of changing for 10x. In addition, it was a holiday
> time, the system was not used for the next few days, giving the oil plenty
> of time. So, it was a coincidence of several factors, but it can happen
> again.
>
> I think, hearing so many positive opinions about hair elastics, I will
> give them the 2nd chance in our facility. Aqua-Stop in my opinion is
> overpriced, and we do not currently do any experiments under perfusion to
> justify such purchase (this may change in the future). However, I would not
> like to give up on the protective window strategy. I think placing a glass
> element in the infinity space is not unusual. It would function as the last
> stronghold against the oil. And it would be considerably cheaper than
> Aqua-Stop. I would of course test PSF. If PSF is not affected, I guess the
> protective window is OK with imaging. So, if you still have some advice
> which one would be most suitable, I would appreciate it.
>
> Best,
> Tomasz
>
> Tomasz Wegierski, PhD
> International Institute of Molecular and Cell Biology
> Trojdena 4, 02-109 Warsaw, POLAND
> tel: +48-22 597 0763
> fax: +48 22 597 0715
>
http://www.iimcb.gov.pl/> ________________________________
> From: Confocal Microscopy List <
[hidden email]> on
> behalf of Frohlich, Victoria <
[hidden email]>
> Sent: Tuesday, October 27, 2020 4:38 PM
> To:
[hidden email] <
[hidden email]>
> Subject: Re: Protective window for a confocal microscope?
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
>
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> *****
>
> Just my 2 cents.
> Scrunchies work well but are not fool-proof. Cotton not polyester
> material are more absorbent.
> In addition to scrunchies I suggest wrapping the lens barrel near the nose
> of the objective with plumbers tape to prevent oil from seeping into the
> barrel. It can be done so that the lens retraction still functions. Then
> once the lens is on the nosepiece of the scope the plumbers tape can be
> wrapped there as well.
>
> I've also used thin rubber sheets or parafilm sheets, as well as filter
> paper (thanks Mike S.!), to draw oil away from the lens and nosepiece.
>
> Best method, however, is to teach users proper oil application and ensure
> they clean up after themselves.
>
> Regards,
> Vickie
>
> Victoria Centonze Frohlich, PhD
> Director, Light Microscopy Shared Resource
> Cell & Tissue Imaging Center
> St. Jude Children’s Research Hospital
> 262 Danny Thomas Place
> MS 312
> Memphis, TN 38105
>
> Office: D1052D
> Phone and Digital Fax: 1-901-595-2536
> Cell: 1-901-907-2596
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> Facility Phone: 1-901-595-3439
>
>
http://home.stjude.org/cell-tissue-imaging/Pages/default.aspx>
> Facility Acknowledgement for publications:
> “Images were acquired at the Cell & Tissue Imaging Center which is
> supported by SJCRH and NCI P30 CA021765.”
>
> -----Original Message-----
> From: Confocal Microscopy List <
[hidden email]> On
> Behalf Of Michael Stanley
> Sent: Tuesday, October 27, 2020 8:19 AM
> To:
[hidden email]
> Subject: Re: Protective window for a confocal microscope?
>
> Caution: External Sender
>
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
>
>
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> *****
>
> in the 'old days' in the core we used to cut filter paper and then bore a
> hole with a cork-bore slightly smaller than the lens. slide 2-3 (or as
> many as will fit) layers of the filter paper over the objective. this will
> not stop a "flood" of oil (which sounds very possible for some) but it will
> hold much more than you would think. and, the most important feature of
> this little trick is that the oil will show very quickly on the paper and
> hopefully will trigger a response.!
>
> some of the darker filter papers will show the oil better, but this will
> vary by manufacturer.
>
> no commercial interest here, just trying to protect all the optics in the
> system.
>
> michael
>
> c. michael stanley, phd
> senior applications scientist
> chroma technology corp.
> 10 imtec lane
> bellows falls, vt 05101
>
[hidden email]
>
[hidden email]
>
>
> -----Original Message-----
> From: Confocal Microscopy List <
[hidden email]> On
> Behalf Of Gary Laevsky
> Sent: Tuesday, October 27, 2020 10:19 AM
> To:
[hidden email]
> Subject: Re: Protective window for a confocal microscope?
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
>
>
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> *****
>
> The hair ties work great with objectives that have enough of a neck for
> the tie to sit on, next to the barrel.
>
> Our 40X objective (most frequently used) has no/very small neck, so the
> hair ties don't work.
>
> I fairly regularly (bi-weekly) have to take the objective off, store it in
> it's case upside down, and put it in a 37C oven. Ridiculous, in a bad way,
> how much oil comes out. The 40X also doesn't have "lip" that acts as a bit
> of a reservoir (unlike the 60 and 100).
>
> On one of my heavily used systems, I have to go so far as to dismantle the
> top (stage, objective turret, and fluorescent turret) to get to the piece
> of glass that prevents leakage into the body (amazingly awesome feature of
> the stand). Takes me 15 minutes. Although I shouldn't have to do that ...
>
> On Tue, Oct 27, 2020 at 9:13 AM Cammer, Michael <
>
[hidden email]> wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> >
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> > *****
> >
> > Aquastop works well for big spills when installed correctly and has
> > proven effective on some of our Zeiss scopes. But we did have a user
> > who spilled over or around it on our Zeiss 710, so it is not foolproof.
> >
> >
> > Hair elastics are great for the oil.
> >
> >
> > Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory
> >
> > NYU Langone Health, 540 First Avenue, SK2 Microscopy Suite, New York,
> > NY
> > 10016
> >
> >
[hidden email]<mailto:
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> >
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> >
> > ________________________________
> > From: Confocal Microscopy List <
[hidden email]> on
> > behalf of Doug Richardson <
[hidden email]>
> > Sent: Tuesday, October 27, 2020 7:41:45 AM
> > To:
[hidden email]
> > Subject: Re: Protective window for a confocal microscope?
> >
> > [EXTERNAL]
> >
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> >
> >
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> > Zfc04rx3ExJHeIIZuCs&r=E0xNnPAQpUbDiPlC50tp7rW2nBkvV7fujQf0RknE5bU&m=ke
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> > Post images on
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> > and include the link in your posting.
> > *****
> >
> > I would highly recommend the aquastop system from Zeiss. It is
> > overpriced for some pieces of plastic, but it forms a very tight seal
> > and pays for itself many times over. It is far superior to hair
> > elastics/glove fingers/condoms which we used in the past.
> >
> > Doug
> >
> > Get Outlook for Android
> >
> > ________________________________
> > From: Confocal Microscopy List <
[hidden email]> on
> > behalf of Tomek Węgierski <
[hidden email]>
> > Sent: Tuesday, October 27, 2020 7:12:10 AM
> > To:
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> > <
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> > Subject: Protective window for a confocal microscope?
> >
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> >
> >
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> > and include the link in your posting.
> > *****
> >
> > Dear All,
> > I am considering a purchase of a protective window for our Zeiss
> > LSM800 and I would like to hear your advice whether this is a good
> > idea, and if yes, which one to buy.
> > The reason is our LSM800 is on an inverted stand and we already got a
> > leakage of oil once which went down onto Optovar. And this was despite
> > my regular checks of objectives (both sides) for leaking oil and
> > extensive education of users on how important it is not to use too
> > much oil and to clean lenses, especially on inverted stands.
> > Zeiss Observer has a dummy slider with an opening for 32 mm filters. I
> > hope, a protective window there should stop the oil, if the leakage
> > happens again.
> > 1) do you think placing a protective window in a confocal microscope
> > is a good idea or not really, because the imaging will be affected?
> > This would be in infinity space, so I guess it should not be
> > detrimental....
> > 2) which characteristics of protective window I should take into
> > consideration (substrate, thickness, coating, flatness, surface
> > quality, parallelism)? Currently I am inclined into protective glasses
> > by Edmund Optics such as 1-mm thick MgF2-coated Sapphire window, which
> > has high transmission, or 2-mm thick MgF2-coated B270 window, which
> > has a bit worse transmission but better surface parameters (and is
> > much cheaper).
> > best regards,
> > Tomasz
> >
> >
> > Tomasz Wegierski, PhD
> > International Institute of Molecular and Cell Biology Trojdena 4,
> > 02-109 Warsaw, POLAND
> > tel: +48-22 597 0763
> > fax: +48 22 597 0715
> >
> >
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> >
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> --
> Best,
>
> Gary Laevsky, Ph.D.
> Director, Confocal Imaging Facility
> Nikon Center of Excellence
> Co-Founder, North Atlantic Microscopy Society (NAMS)
>
>
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