http://confocal-microscopy-list.275.s1.nabble.com/Protective-window-for-a-confocal-microscope-tp7591445p7591463.html
> *****
> To join, leave or search the confocal microscopy listserv, go to:
>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> Post images on
http://www.imgur.com and include the link in your posting.
> *****
>
> Hi Tomasz,
>
> back to your question. The filter holder is there to hold a filter. Any
> decent glass or fused silica AR window should do the trick.
> As far as I remember, the hole is quite big, around 30 mm diameter (bigger
> than the standard 25 mm filters), which may limit your options.
>
> Most filters and windows have specified surface flatness (reflected wave
> error), but it's actually transmitted wave error (TWE) that matters here.
> Usually, windows with single digit flatness (in lambda or fringe units)
> will have TWE << 1/5 lambda, and should be OK.
>
> Thicker substrate (2 - 3 mm) usually means precision optics, and is a safe
> bet. Hard coating is a must, if you want to wipe oil off of it regularly.
> All reputable brands use hard coatings these days, 1% loss (per pass) is
> OK.
>
> Sapphire is hard to work with and comes with a price premium. Fused silica,
> e.g.
>
>
https://www.edmundoptics.com/p/30mm-dia-vis-nir-coated-1lambda-fused-silica-window/10158/> (no commercial interest) looks like a good option, but BK-7 will work well,
> too.
>
> If you happen to have a piece of AR-coated glass at hand, just put it there
> and look at some beads. If you're not able to tell the difference, you're
> good to go. You can even try a glass coverslip, the 4% loss (the excitation
> loss is not an issue, you can always crank up the laser) will be hardly
> noticeable. Coverslips are not polished, so the TWE is generally bad, but
> with some luck you can find a good one in the stack...
>
> Best, zdenek
>
>
> On Tue, Oct 27, 2020 at 1:18 PM Tomek Węgierski <
[hidden email]>
> wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> >
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> > Post images on
http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> > Dear All,
> >
> > Thank you very much for so many responses, although the discussion went a
> > bit into a direction that I did not anticipate ??
> > But yes, it is all about preventing oil from getting into the microscope.
> > Our 40x/1.3 Fluar lens takes a lot of oil and I frequently have to clean
> > its bottom side from oil. In contrast, I never saw any oil on the back of
> > 63x/1.4 even though it is used as frequently as the 40x.
> >
> > I have to admit I have been skeptical about hair elastics, because when
> > they saturate with oil they will just let it go. IMHO, it is replacing
> "oil
> > cleaning" approach with "oil buffering" approach. The "cleaning" approach
> > has worked for us for many years. It failed when an unexperienced user
> (who
> > apparently did not take seriously what I was talking during the training)
> > made two mistakes: did not clean the lens and let it stay in the optic
> path
> > after the work instead of changing for 10x. In addition, it was a holiday
> > time, the system was not used for the next few days, giving the oil
> plenty
> > of time. So, it was a coincidence of several factors, but it can happen
> > again.
> >
> > I think, hearing so many positive opinions about hair elastics, I will
> > give them the 2nd chance in our facility. Aqua-Stop in my opinion is
> > overpriced, and we do not currently do any experiments under perfusion to
> > justify such purchase (this may change in the future). However, I would
> not
> > like to give up on the protective window strategy. I think placing a
> glass
> > element in the infinity space is not unusual. It would function as the
> last
> > stronghold against the oil. And it would be considerably cheaper than
> > Aqua-Stop. I would of course test PSF. If PSF is not affected, I guess
> the
> > protective window is OK with imaging. So, if you still have some advice
> > which one would be most suitable, I would appreciate it.
> >
> > Best,
> > Tomasz
> >
> > Tomasz Wegierski, PhD
> > International Institute of Molecular and Cell Biology
> > Trojdena 4, 02-109 Warsaw, POLAND
> > tel: +48-22 597 0763
> > fax: +48 22 597 0715
> >
http://www.iimcb.gov.pl/> > ________________________________
> > From: Confocal Microscopy List <
[hidden email]> on
> > behalf of Frohlich, Victoria <
[hidden email]>
> > Sent: Tuesday, October 27, 2020 4:38 PM
> > To:
[hidden email] <
[hidden email]>
> > Subject: Re: Protective window for a confocal microscope?
> >
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> >
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> > Post images on
http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> > Just my 2 cents.
> > Scrunchies work well but are not fool-proof. Cotton not polyester
> > material are more absorbent.
> > In addition to scrunchies I suggest wrapping the lens barrel near the
> nose
> > of the objective with plumbers tape to prevent oil from seeping into the
> > barrel. It can be done so that the lens retraction still functions.
> Then
> > once the lens is on the nosepiece of the scope the plumbers tape can be
> > wrapped there as well.
> >
> > I've also used thin rubber sheets or parafilm sheets, as well as filter
> > paper (thanks Mike S.!), to draw oil away from the lens and nosepiece.
> >
> > Best method, however, is to teach users proper oil application and ensure
> > they clean up after themselves.
> >
> > Regards,
> > Vickie
> >
> > Victoria Centonze Frohlich, PhD
> > Director, Light Microscopy Shared Resource
> > Cell & Tissue Imaging Center
> > St. Jude Children’s Research Hospital
> > 262 Danny Thomas Place
> > MS 312
> > Memphis, TN 38105
> >
> > Office: D1052D
> > Phone and Digital Fax: 1-901-595-2536
> > Cell: 1-901-907-2596
> > Paper Fax: 1-901-595-2909
> > Email:
[hidden email]
> >
> > CTIC-LM Location: D1055 and D1056
> > Facility Phone: 1-901-595-3439
> >
> >
http://home.stjude.org/cell-tissue-imaging/Pages/default.aspx> >
> > Facility Acknowledgement for publications:
> > “Images were acquired at the Cell & Tissue Imaging Center which is
> > supported by SJCRH and NCI P30 CA021765.”
> >
> > -----Original Message-----
> > From: Confocal Microscopy List <
[hidden email]> On
> > Behalf Of Michael Stanley
> > Sent: Tuesday, October 27, 2020 8:19 AM
> > To:
[hidden email]
> > Subject: Re: Protective window for a confocal microscope?
> >
> > Caution: External Sender
> >
> >
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> >
> >
>
https://nam11.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.umn.edu%2Fcgi-bin%2Fwa%3FA0%3Dconfocalmicroscopy&data=04%7C01%7Cvictoria.frohlich%40STJUDE.ORG%7C6b89a1a0f4cc42f5937e08d87a8d1047%7C22340fa892264871b677d3b3e377af72%7C0%7C0%7C637394093573449006%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=WiaypCuZUL5s03B%2Br1rcmMLyOazUGYLj%2BmW7hRFcyIU%3D&reserved=0> > Post images on
> >
>
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> > *****
> >
> > in the 'old days' in the core we used to cut filter paper and then bore a
> > hole with a cork-bore slightly smaller than the lens. slide 2-3 (or as
> > many as will fit) layers of the filter paper over the objective. this
> will
> > not stop a "flood" of oil (which sounds very possible for some) but it
> will
> > hold much more than you would think. and, the most important feature of
> > this little trick is that the oil will show very quickly on the paper and
> > hopefully will trigger a response.!
> >
> > some of the darker filter papers will show the oil better, but this will
> > vary by manufacturer.
> >
> > no commercial interest here, just trying to protect all the optics in the
> > system.
> >
> > michael
> >
> > c. michael stanley, phd
> > senior applications scientist
> > chroma technology corp.
> > 10 imtec lane
> > bellows falls, vt 05101
> >
[hidden email]
> >
[hidden email]
> >
> >
> > -----Original Message-----
> > From: Confocal Microscopy List <
[hidden email]> On
> > Behalf Of Gary Laevsky
> > Sent: Tuesday, October 27, 2020 10:19 AM
> > To:
[hidden email]
> > Subject: Re: Protective window for a confocal microscope?
> >
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> >
> >
>
https://nam11.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.umn.edu%2Fcgi-bin%2Fwa%3FA0%3Dconfocalmicroscopy&data=04%7C01%7Cvictoria.frohlich%40STJUDE.ORG%7C6b89a1a0f4cc42f5937e08d87a8d1047%7C22340fa892264871b677d3b3e377af72%7C0%7C0%7C637394093573449006%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=WiaypCuZUL5s03B%2Br1rcmMLyOazUGYLj%2BmW7hRFcyIU%3D&reserved=0> > Post images on
> >
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> > *****
> >
> > The hair ties work great with objectives that have enough of a neck for
> > the tie to sit on, next to the barrel.
> >
> > Our 40X objective (most frequently used) has no/very small neck, so the
> > hair ties don't work.
> >
> > I fairly regularly (bi-weekly) have to take the objective off, store it
> in
> > it's case upside down, and put it in a 37C oven. Ridiculous, in a bad
> way,
> > how much oil comes out. The 40X also doesn't have "lip" that acts as a
> bit
> > of a reservoir (unlike the 60 and 100).
> >
> > On one of my heavily used systems, I have to go so far as to dismantle
> the
> > top (stage, objective turret, and fluorescent turret) to get to the piece
> > of glass that prevents leakage into the body (amazingly awesome feature
> of
> > the stand). Takes me 15 minutes. Although I shouldn't have to do that
> ...
> >
> > On Tue, Oct 27, 2020 at 9:13 AM Cammer, Michael <
> >
[hidden email]> wrote:
> >
> > > *****
> > > To join, leave or search the confocal microscopy listserv, go to:
> > >
https://nam11.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists> > > .umn.edu%2Fcgi-bin%2Fwa%3FA0%3Dconfocalmicroscopy&data=04%7C01%7Cv
> > > ictoria.frohlich%40STJUDE.ORG%7C6b89a1a0f4cc42f5937e08d87a8d1047%7C223
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> > > CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXV
> > > CI6Mn0%3D%7C1000&sdata=q2oFBgmsbEfra%2BIBWB6i8xwEJ%2B8SF%2F59WSo3Y
> > > %2BKvFKg%3D&reserved=0 Post images on
> > >
> >
>
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> > > *****
> > >
> > > Aquastop works well for big spills when installed correctly and has
> > > proven effective on some of our Zeiss scopes. But we did have a user
> > > who spilled over or around it on our Zeiss 710, so it is not foolproof.
> > >
> > >
> > > Hair elastics are great for the oil.
> > >
> > >
> > > Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory
> > >
> > > NYU Langone Health, 540 First Avenue, SK2 Microscopy Suite, New York,
> > > NY
> > > 10016
> > >
> > >
[hidden email]<mailto:
[hidden email]>
> > >
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> > >
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> > >
> > > Voice direct only, no text or messages: 1-914-309-3270 and
> > > 1-646-501-0567
> > >
> > >
> > >
> > > ________________________________
> > > From: Confocal Microscopy List <
[hidden email]> on
> > > behalf of Doug Richardson <
[hidden email]>
> > > Sent: Tuesday, October 27, 2020 7:41:45 AM
> > > To:
[hidden email]
> > > Subject: Re: Protective window for a confocal microscope?
> > >
> > > [EXTERNAL]
> > >
> > > *****
> > > To join, leave or search the confocal microscopy listserv, go to:
> > >
> > >
https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-> > > 2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIFBA&c=j5oPpO0eBH1iio48DtsedeEl
> > > Zfc04rx3ExJHeIIZuCs&r=E0xNnPAQpUbDiPlC50tp7rW2nBkvV7fujQf0RknE5bU&m=ke
> > > g1PHlpRuZ6hHabv7CvVJ2rsWSRa_2pkARNBtS-dFQ&s=k-eCFZaqV2J3Nbh8c16znCG7uP
> > > Pgwni6bBeSRyqYRAE&e=
> > > Post images on
> > >
https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=Dw> > > IFBA&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=E0xNnPAQpUbDiPlC5
> > > 0tp7rW2nBkvV7fujQf0RknE5bU&m=keg1PHlpRuZ6hHabv7CvVJ2rsWSRa_2pkARNBtS-d
> > > FQ&s=o9KAzzxXBHkEPM_YiZYo9mb7m2x6GjWRoUHh-BzcNSg&e=
> > > and include the link in your posting.
> > > *****
> > >
> > > I would highly recommend the aquastop system from Zeiss. It is
> > > overpriced for some pieces of plastic, but it forms a very tight seal
> > > and pays for itself many times over. It is far superior to hair
> > > elastics/glove fingers/condoms which we used in the past.
> > >
> > > Doug
> > >
> > > Get Outlook for Android
> > >
> > > ________________________________
> > > From: Confocal Microscopy List <
[hidden email]> on
> > > behalf of Tomek Węgierski <
[hidden email]>
> > > Sent: Tuesday, October 27, 2020 7:12:10 AM
> > > To:
[hidden email]
> > > <
[hidden email]>
> > > Subject: Protective window for a confocal microscope?
> > >
> > > *****
> > > To join, leave or search the confocal microscopy listserv, go to:
> > >
> > >
https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-> > > 2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIFBA&c=j5oPpO0eBH1iio48DtsedeEl
> > > Zfc04rx3ExJHeIIZuCs&r=E0xNnPAQpUbDiPlC50tp7rW2nBkvV7fujQf0RknE5bU&m=ke
> > > g1PHlpRuZ6hHabv7CvVJ2rsWSRa_2pkARNBtS-dFQ&s=k-eCFZaqV2J3Nbh8c16znCG7uP
> > > Pgwni6bBeSRyqYRAE&e=
> > > Post images on
> > >
https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=Dw> > > IFBA&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=E0xNnPAQpUbDiPlC5
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> > > FQ&s=o9KAzzxXBHkEPM_YiZYo9mb7m2x6GjWRoUHh-BzcNSg&e=
> > > and include the link in your posting.
> > > *****
> > >
> > > Dear All,
> > > I am considering a purchase of a protective window for our Zeiss
> > > LSM800 and I would like to hear your advice whether this is a good
> > > idea, and if yes, which one to buy.
> > > The reason is our LSM800 is on an inverted stand and we already got a
> > > leakage of oil once which went down onto Optovar. And this was despite
> > > my regular checks of objectives (both sides) for leaking oil and
> > > extensive education of users on how important it is not to use too
> > > much oil and to clean lenses, especially on inverted stands.
> > > Zeiss Observer has a dummy slider with an opening for 32 mm filters. I
> > > hope, a protective window there should stop the oil, if the leakage
> > > happens again.
> > > 1) do you think placing a protective window in a confocal microscope
> > > is a good idea or not really, because the imaging will be affected?
> > > This would be in infinity space, so I guess it should not be
> > > detrimental....
> > > 2) which characteristics of protective window I should take into
> > > consideration (substrate, thickness, coating, flatness, surface
> > > quality, parallelism)? Currently I am inclined into protective glasses
> > > by Edmund Optics such as 1-mm thick MgF2-coated Sapphire window, which
> > > has high transmission, or 2-mm thick MgF2-coated B270 window, which
> > > has a bit worse transmission but better surface parameters (and is
> > > much cheaper).
> > > best regards,
> > > Tomasz
> > >
> > >
> > > Tomasz Wegierski, PhD
> > > International Institute of Molecular and Cell Biology Trojdena 4,
> > > 02-109 Warsaw, POLAND
> > > tel: +48-22 597 0763
> > > fax: +48 22 597 0715
> > >
> > >
https://urldefense.proofpoint.com/v2/url?u=http-3A__www.iimcb.gov.pl_&> > > d=DwIFBA&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=E0xNnPAQpUbDi
> > > PlC50tp7rW2nBkvV7fujQf0RknE5bU&m=keg1PHlpRuZ6hHabv7CvVJ2rsWSRa_2pkARNB
> > > tS-dFQ&s=u7qHptX43byvVfMe-Zn04ev5KF4-ToW1ss01pq0eVtM&e=
> > >
> > > ------------------------------------------------------------
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> > >
> >
> >
> > --
> > Best,
> >
> > Gary Laevsky, Ph.D.
> > Director, Confocal Imaging Facility
> > Nikon Center of Excellence
> > Co-Founder, North Atlantic Microscopy Society (NAMS)
> >
> >
>
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>
> --
> --
> Zdenek Svindrych, Ph.D.
> Research Scientist - Microscopy Imaging Specialist
> Department of Biochemistry and Cell Biology
> Geisel School of Medicine at Dartmouth
>
Dept. of Molecular Biology