http://confocal-microscopy-list.275.s1.nabble.com/Protective-window-for-a-confocal-microscope-tp7591445p7591468.html
filter in the infinity space. And, indeed, most flat surfaces (windows,
> *****
> To join, leave or search the confocal microscopy listserv, go to:
>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> Post images on
http://www.imgur.com and include the link in your posting.
> *****
>
> Hi All,
>
> Staying on the question, in regards to putting a piece of glass in that
> slider (which would make our lives easier), should it be placed on an
> angle, to minimize reflections? But, will that increase aberration?
>
> Good question Tomasz.
>
> On Tue, Oct 27, 2020 at 7:46 PM Zdenek Svindrych <
[hidden email]>
> wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> >
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> > Post images on
http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> > Hi Tomasz,
> >
> > back to your question. The filter holder is there to hold a filter. Any
> > decent glass or fused silica AR window should do the trick.
> > As far as I remember, the hole is quite big, around 30 mm diameter
> (bigger
> > than the standard 25 mm filters), which may limit your options.
> >
> > Most filters and windows have specified surface flatness (reflected wave
> > error), but it's actually transmitted wave error (TWE) that matters here.
> > Usually, windows with single digit flatness (in lambda or fringe units)
> > will have TWE << 1/5 lambda, and should be OK.
> >
> > Thicker substrate (2 - 3 mm) usually means precision optics, and is a
> safe
> > bet. Hard coating is a must, if you want to wipe oil off of it regularly.
> > All reputable brands use hard coatings these days, 1% loss (per pass) is
> > OK.
> >
> > Sapphire is hard to work with and comes with a price premium. Fused
> silica,
> > e.g.
> >
> >
>
https://www.edmundoptics.com/p/30mm-dia-vis-nir-coated-1lambda-fused-silica-window/10158/> > (no commercial interest) looks like a good option, but BK-7 will work
> well,
> > too.
> >
> > If you happen to have a piece of AR-coated glass at hand, just put it
> there
> > and look at some beads. If you're not able to tell the difference, you're
> > good to go. You can even try a glass coverslip, the 4% loss (the
> excitation
> > loss is not an issue, you can always crank up the laser) will be hardly
> > noticeable. Coverslips are not polished, so the TWE is generally bad, but
> > with some luck you can find a good one in the stack...
> >
> > Best, zdenek
> >
> >
> > On Tue, Oct 27, 2020 at 1:18 PM Tomek Węgierski <
[hidden email]>
> > wrote:
> >
> > > *****
> > > To join, leave or search the confocal microscopy listserv, go to:
> > >
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> > > Post images on
http://www.imgur.com and include the link in your
> > posting.
> > > *****
> > >
> > > Dear All,
> > >
> > > Thank you very much for so many responses, although the discussion
> went a
> > > bit into a direction that I did not anticipate ??
> > > But yes, it is all about preventing oil from getting into the
> microscope.
> > > Our 40x/1.3 Fluar lens takes a lot of oil and I frequently have to
> clean
> > > its bottom side from oil. In contrast, I never saw any oil on the back
> of
> > > 63x/1.4 even though it is used as frequently as the 40x.
> > >
> > > I have to admit I have been skeptical about hair elastics, because when
> > > they saturate with oil they will just let it go. IMHO, it is replacing
> > "oil
> > > cleaning" approach with "oil buffering" approach. The "cleaning"
> approach
> > > has worked for us for many years. It failed when an unexperienced user
> > (who
> > > apparently did not take seriously what I was talking during the
> training)
> > > made two mistakes: did not clean the lens and let it stay in the optic
> > path
> > > after the work instead of changing for 10x. In addition, it was a
> holiday
> > > time, the system was not used for the next few days, giving the oil
> > plenty
> > > of time. So, it was a coincidence of several factors, but it can happen
> > > again.
> > >
> > > I think, hearing so many positive opinions about hair elastics, I will
> > > give them the 2nd chance in our facility. Aqua-Stop in my opinion is
> > > overpriced, and we do not currently do any experiments under perfusion
> to
> > > justify such purchase (this may change in the future). However, I would
> > not
> > > like to give up on the protective window strategy. I think placing a
> > glass
> > > element in the infinity space is not unusual. It would function as the
> > last
> > > stronghold against the oil. And it would be considerably cheaper than
> > > Aqua-Stop. I would of course test PSF. If PSF is not affected, I guess
> > the
> > > protective window is OK with imaging. So, if you still have some advice
> > > which one would be most suitable, I would appreciate it.
> > >
> > > Best,
> > > Tomasz
> > >
> > > Tomasz Wegierski, PhD
> > > International Institute of Molecular and Cell Biology
> > > Trojdena 4, 02-109 Warsaw, POLAND
> > > tel: +48-22 597 0763
> > > fax: +48 22 597 0715
> > >
http://www.iimcb.gov.pl/> > > ________________________________
> > > From: Confocal Microscopy List <
[hidden email]> on
> > > behalf of Frohlich, Victoria <
[hidden email]>
> > > Sent: Tuesday, October 27, 2020 4:38 PM
> > > To:
[hidden email] <
[hidden email]
> >
> > > Subject: Re: Protective window for a confocal microscope?
> > >
> > > *****
> > > To join, leave or search the confocal microscopy listserv, go to:
> > >
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> > > Post images on
http://www.imgur.com and include the link in your
> > posting.
> > > *****
> > >
> > > Just my 2 cents.
> > > Scrunchies work well but are not fool-proof. Cotton not polyester
> > > material are more absorbent.
> > > In addition to scrunchies I suggest wrapping the lens barrel near the
> > nose
> > > of the objective with plumbers tape to prevent oil from seeping into
> the
> > > barrel. It can be done so that the lens retraction still functions.
> > Then
> > > once the lens is on the nosepiece of the scope the plumbers tape can be
> > > wrapped there as well.
> > >
> > > I've also used thin rubber sheets or parafilm sheets, as well as filter
> > > paper (thanks Mike S.!), to draw oil away from the lens and nosepiece.
> > >
> > > Best method, however, is to teach users proper oil application and
> ensure
> > > they clean up after themselves.
> > >
> > > Regards,
> > > Vickie
> > >
> > > Victoria Centonze Frohlich, PhD
> > > Director, Light Microscopy Shared Resource
> > > Cell & Tissue Imaging Center
> > > St. Jude Children’s Research Hospital
> > > 262 Danny Thomas Place
> > > MS 312
> > > Memphis, TN 38105
> > >
> > > Office: D1052D
> > > Phone and Digital Fax: 1-901-595-2536
> > > Cell: 1-901-907-2596
> > > Paper Fax: 1-901-595-2909
> > > Email:
[hidden email]
> > >
> > > CTIC-LM Location: D1055 and D1056
> > > Facility Phone: 1-901-595-3439
> > >
> > >
http://home.stjude.org/cell-tissue-imaging/Pages/default.aspx> > >
> > > Facility Acknowledgement for publications:
> > > “Images were acquired at the Cell & Tissue Imaging Center which is
> > > supported by SJCRH and NCI P30 CA021765.”
> > >
> > > -----Original Message-----
> > > From: Confocal Microscopy List <
[hidden email]> On
> > > Behalf Of Michael Stanley
> > > Sent: Tuesday, October 27, 2020 8:19 AM
> > > To:
[hidden email]
> > > Subject: Re: Protective window for a confocal microscope?
> > >
> > > Caution: External Sender
> > >
> > >
> > > *****
> > > To join, leave or search the confocal microscopy listserv, go to:
> > >
> > >
> >
>
https://nam11.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.umn.edu%2Fcgi-bin%2Fwa%3FA0%3Dconfocalmicroscopy&data=04%7C01%7Cvictoria.frohlich%40STJUDE.ORG%7C6b89a1a0f4cc42f5937e08d87a8d1047%7C22340fa892264871b677d3b3e377af72%7C0%7C0%7C637394093573449006%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=WiaypCuZUL5s03B%2Br1rcmMLyOazUGYLj%2BmW7hRFcyIU%3D&reserved=0> > > Post images on
> > >
> >
>
https://nam11.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.imgur.com%2F&data=04%7C01%7Cvictoria.frohlich%40STJUDE.ORG%7C6b89a1a0f4cc42f5937e08d87a8d1047%7C22340fa892264871b677d3b3e377af72%7C0%7C0%7C637394093573449006%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=DKQAcpfaPdTDony5B7X%2Fdi%2BCVChjA7voJxvlDKq9suo%3D&reserved=0> > > and include the link in your posting.
> > > *****
> > >
> > > in the 'old days' in the core we used to cut filter paper and then
> bore a
> > > hole with a cork-bore slightly smaller than the lens. slide 2-3 (or as
> > > many as will fit) layers of the filter paper over the objective. this
> > will
> > > not stop a "flood" of oil (which sounds very possible for some) but it
> > will
> > > hold much more than you would think. and, the most important feature
> of
> > > this little trick is that the oil will show very quickly on the paper
> and
> > > hopefully will trigger a response.!
> > >
> > > some of the darker filter papers will show the oil better, but this
> will
> > > vary by manufacturer.
> > >
> > > no commercial interest here, just trying to protect all the optics in
> the
> > > system.
> > >
> > > michael
> > >
> > > c. michael stanley, phd
> > > senior applications scientist
> > > chroma technology corp.
> > > 10 imtec lane
> > > bellows falls, vt 05101
> > >
[hidden email]
> > >
[hidden email]
> > >
> > >
> > > -----Original Message-----
> > > From: Confocal Microscopy List <
[hidden email]> On
> > > Behalf Of Gary Laevsky
> > > Sent: Tuesday, October 27, 2020 10:19 AM
> > > To:
[hidden email]
> > > Subject: Re: Protective window for a confocal microscope?
> > >
> > > *****
> > > To join, leave or search the confocal microscopy listserv, go to:
> > >
> > >
> >
>
https://nam11.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.umn.edu%2Fcgi-bin%2Fwa%3FA0%3Dconfocalmicroscopy&data=04%7C01%7Cvictoria.frohlich%40STJUDE.ORG%7C6b89a1a0f4cc42f5937e08d87a8d1047%7C22340fa892264871b677d3b3e377af72%7C0%7C0%7C637394093573449006%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=WiaypCuZUL5s03B%2Br1rcmMLyOazUGYLj%2BmW7hRFcyIU%3D&reserved=0> > > Post images on
> > >
> >
>
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> > > *****
> > >
> > > The hair ties work great with objectives that have enough of a neck for
> > > the tie to sit on, next to the barrel.
> > >
> > > Our 40X objective (most frequently used) has no/very small neck, so the
> > > hair ties don't work.
> > >
> > > I fairly regularly (bi-weekly) have to take the objective off, store it
> > in
> > > it's case upside down, and put it in a 37C oven. Ridiculous, in a bad
> > way,
> > > how much oil comes out. The 40X also doesn't have "lip" that acts as a
> > bit
> > > of a reservoir (unlike the 60 and 100).
> > >
> > > On one of my heavily used systems, I have to go so far as to dismantle
> > the
> > > top (stage, objective turret, and fluorescent turret) to get to the
> piece
> > > of glass that prevents leakage into the body (amazingly awesome feature
> > of
> > > the stand). Takes me 15 minutes. Although I shouldn't have to do that
> > ...
> > >
> > > On Tue, Oct 27, 2020 at 9:13 AM Cammer, Michael <
> > >
[hidden email]> wrote:
> > >
> > > > *****
> > > > To join, leave or search the confocal microscopy listserv, go to:
> > > >
>
https://nam11.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists> > > > .umn.edu
> %2Fcgi-bin%2Fwa%3FA0%3Dconfocalmicroscopy&data=04%7C01%7Cv
> > > > ictoria.frohlich%40STJUDE.ORG
> %7C6b89a1a0f4cc42f5937e08d87a8d1047%7C223
> > > >
> 40fa892264871b677d3b3e377af72%7C0%7C0%7C637394093573459000%7CUnknown%7
> > > >
> CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXV
> > > >
> CI6Mn0%3D%7C1000&sdata=q2oFBgmsbEfra%2BIBWB6i8xwEJ%2B8SF%2F59WSo3Y
> > > > %2BKvFKg%3D&reserved=0 Post images on
> > > >
> > >
> >
>
https://nam11.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.imgur.com%2F&data=04%7C01%7Cvictoria.frohlich%40STJUDE.ORG%7C6b89a1a0f4cc42f5937e08d87a8d1047%7C22340fa892264871b677d3b3e377af72%7C0%7C0%7C637394093573459000%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=yhWCT39l%2FiZJ9i8H0f4EM7MIstxliSvBVsjVQVNN8cI%3D&reserved=0> > > and include the link in your posting.
> > > > *****
> > > >
> > > > Aquastop works well for big spills when installed correctly and has
> > > > proven effective on some of our Zeiss scopes. But we did have a user
> > > > who spilled over or around it on our Zeiss 710, so it is not
> foolproof.
> > > >
> > > >
> > > > Hair elastics are great for the oil.
> > > >
> > > >
> > > > Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory
> > > >
> > > > NYU Langone Health, 540 First Avenue, SK2 Microscopy Suite, New York,
> > > > NY
> > > > 10016
> > > >
> > > >
[hidden email]<mailto:
[hidden email]>
> > > >
>
https://nam11.safelinks.protection.outlook.com/?url=http%3A%2F%2Fnyulm> > > > c.org%2Fmicros&data=04%7C01%7Cvictoria.frohlich%40STJUDE.ORG
> %7C6b8
> > > >
> 9a1a0f4cc42f5937e08d87a8d1047%7C22340fa892264871b677d3b3e377af72%7C0%7
> > > >
> C0%7C637394093573459000%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLC
> > > >
> JQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=Ud4O3pAe
> > > > 6c4HlzFgcjkQBvmAAz45ILNKOj8mnQScAsg%3D&reserved=0
> > > >
>
https://nam11.safelinks.protection.outlook.com/?url=http%3A%2F%2Fmicro> > > > scopynotes.com%2F&data=04%7C01%7Cvictoria.frohlich%40STJUDE.ORG
> %7C
> > > >
> 6b89a1a0f4cc42f5937e08d87a8d1047%7C22340fa892264871b677d3b3e377af72%7C
> > > >
> 0%7C0%7C637394093573459000%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDA
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> iLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=e6srs
> > > > AN7BHwtFBKtNbXavROHTyX2m0CdnPe3l7VnUPA%3D&reserved=0
> > > >
> > > > Voice direct only, no text or messages: 1-914-309-3270 and
> > > > 1-646-501-0567
> > > >
> > > >
> > > >
> > > > ________________________________
> > > > From: Confocal Microscopy List <
[hidden email]> on
> > > > behalf of Doug Richardson <
[hidden email]>
> > > > Sent: Tuesday, October 27, 2020 7:41:45 AM
> > > > To:
[hidden email]
> > > > Subject: Re: Protective window for a confocal microscope?
> > > >
> > > > [EXTERNAL]
> > > >
> > > > *****
> > > > To join, leave or search the confocal microscopy listserv, go to:
> > > >
> > > >
>
https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-> > > >
> 2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIFBA&c=j5oPpO0eBH1iio48DtsedeEl
> > > >
> Zfc04rx3ExJHeIIZuCs&r=E0xNnPAQpUbDiPlC50tp7rW2nBkvV7fujQf0RknE5bU&m=ke
> > > >
> g1PHlpRuZ6hHabv7CvVJ2rsWSRa_2pkARNBtS-dFQ&s=k-eCFZaqV2J3Nbh8c16znCG7uP
> > > > Pgwni6bBeSRyqYRAE&e=
> > > > Post images on
> > > >
>
https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=Dw> > > >
> IFBA&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=E0xNnPAQpUbDiPlC5
> > > >
> 0tp7rW2nBkvV7fujQf0RknE5bU&m=keg1PHlpRuZ6hHabv7CvVJ2rsWSRa_2pkARNBtS-d
> > > > FQ&s=o9KAzzxXBHkEPM_YiZYo9mb7m2x6GjWRoUHh-BzcNSg&e=
> > > > and include the link in your posting.
> > > > *****
> > > >
> > > > I would highly recommend the aquastop system from Zeiss. It is
> > > > overpriced for some pieces of plastic, but it forms a very tight seal
> > > > and pays for itself many times over. It is far superior to hair
> > > > elastics/glove fingers/condoms which we used in the past.
> > > >
> > > > Doug
> > > >
> > > > Get Outlook for Android
> > > >
> > > > ________________________________
> > > > From: Confocal Microscopy List <
[hidden email]> on
> > > > behalf of Tomek Węgierski <
[hidden email]>
> > > > Sent: Tuesday, October 27, 2020 7:12:10 AM
> > > > To:
[hidden email]
> > > > <
[hidden email]>
> > > > Subject: Protective window for a confocal microscope?
> > > >
> > > > *****
> > > > To join, leave or search the confocal microscopy listserv, go to:
> > > >
> > > >
>
https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-> > > >
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> > > >
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> > > > Pgwni6bBeSRyqYRAE&e=
> > > > Post images on
> > > >
>
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> IFBA&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=E0xNnPAQpUbDiPlC5
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> > > > FQ&s=o9KAzzxXBHkEPM_YiZYo9mb7m2x6GjWRoUHh-BzcNSg&e=
> > > > and include the link in your posting.
> > > > *****
> > > >
> > > > Dear All,
> > > > I am considering a purchase of a protective window for our Zeiss
> > > > LSM800 and I would like to hear your advice whether this is a good
> > > > idea, and if yes, which one to buy.
> > > > The reason is our LSM800 is on an inverted stand and we already got a
> > > > leakage of oil once which went down onto Optovar. And this was
> despite
> > > > my regular checks of objectives (both sides) for leaking oil and
> > > > extensive education of users on how important it is not to use too
> > > > much oil and to clean lenses, especially on inverted stands.
> > > > Zeiss Observer has a dummy slider with an opening for 32 mm filters.
> I
> > > > hope, a protective window there should stop the oil, if the leakage
> > > > happens again.
> > > > 1) do you think placing a protective window in a confocal microscope
> > > > is a good idea or not really, because the imaging will be affected?
> > > > This would be in infinity space, so I guess it should not be
> > > > detrimental....
> > > > 2) which characteristics of protective window I should take into
> > > > consideration (substrate, thickness, coating, flatness, surface
> > > > quality, parallelism)? Currently I am inclined into protective
> glasses
> > > > by Edmund Optics such as 1-mm thick MgF2-coated Sapphire window,
> which
> > > > has high transmission, or 2-mm thick MgF2-coated B270 window, which
> > > > has a bit worse transmission but better surface parameters (and is
> > > > much cheaper).
> > > > best regards,
> > > > Tomasz
> > > >
> > > >
> > > > Tomasz Wegierski, PhD
> > > > International Institute of Molecular and Cell Biology Trojdena 4,
> > > > 02-109 Warsaw, POLAND
> > > > tel: +48-22 597 0763
> > > > fax: +48 22 597 0715
> > > >
> > > >
>
https://urldefense.proofpoint.com/v2/url?u=http-3A__www.iimcb.gov.pl_&> > > >
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> > > >
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> Best,
>
> Gary Laevsky, Ph.D.
> Director, Confocal Imaging Facility
> Nikon Center of Excellence
> Co-Founder, North Atlantic Microscopy Society (NAMS)
>
https://namsmicroscopy.com/> Dept. of Molecular Biology
> Washington Rd.
> Princeton University
> Princeton, New Jersey, 08544-1014
> (O) 609 258 5432
> (C) 508 507 1310
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