Re: Zeiss oil on Leica microscope

Posted by Michael Abanto on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Zeiss-oil-on-Leica-microscope-tp7591810p7591827.html

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just before that topic is closed... as I understand it the issue would be crystallization formed by mixing different oils on the same microscope objective. However, any one oil used without contamination of another would be fine. There might be subtle optimization for the optic/abberations that are brand specific and might only be noticed at high res.


I have never done a test or seen the data for crystallization nor seen the optical differences published.


Is it worth someone publishing a comparison of modern oils on different objectives. It would be great if we could all just buy Cargille for cheap (which I have heard that many companies do and then rebottle) and added specific chemicals as/if needed.


ditto mounting media.


Cheers,


Mike


Michael Abanto, PhD
Head of Microscopy Facility
Department of Biomedicine, Hebelstrasse 20
University Hospital Basel

tel [phone]
http://biomedizin.unibas.ch/services/microscopy-facilities/facility-hebelstrasse/
http://microscopynetwork.unibas.ch
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From: Confocal Microscopy List <[hidden email]> on behalf of Khoroshyy Petro <[hidden email]>
Sent: Monday, February 15, 2021 2:18:45 PM
To: [hidden email]
Subject: Re: Zeiss oil on Leica microscope

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Thanks to everyone for their comments.
While I think it is the same situation, but,  can anyone Any comments on
the use of Immersol W  with non-Zeiss objectives.
Best.
Petro.

On Thu, Feb 11, 2021 at 10:18 AM Antonio Virgilio Failla <[hidden email]>
wrote:

> On 10.02.2021 09:20, Khoroshyy Petro wrote:
>
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>
> Hi, list.
> I was told recently by a Leica engineer that he encountered many Leica
> objectives destroyed by Zeiss oil. I was not able to figure out from him if
> he was pooling my leg (which I think is the case) or was talking seriously.
> Thanks.
> Petro.
>
>
> Hi Petro
> is now 10 years that we are using Zeiss oil on Leica microscopes without
> ojective damages and without the Leica service engineer
>
> <https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=&cad=rja&uact=8&ved=2ahUKEwi_5-TSv-HuAhWzQkEAHRTECf4QFjAAegQIBhAC&url=https%3A%2F%2Fen.wikipedia.org%2Fwiki%2FEngineer&usg=AOvVaw3sD6JZYnJ2ks0fC8YV0Lzk>complaining
> about it.
> Best wishes
> Virgilio
>
> --
> Dr. Antonio Virgilio Failla
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Petro Khoroshyy