Re: Zeiss oil on Leica microscope

Posted by Zdenek Svindrych-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Zeiss-oil-on-Leica-microscope-tp7591810p7591828.html

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Just few quick notes,

Even relatively modern oils may form crystals on long storage. Or some
components may be slightly volatile. Then, over years and years you may
find the refractive index is way off...

Many Cargille oils mix OK. Except for the low RI ones (fluorinated
hydrocarbons). Get an Abbe refractometer to measure the actual RI
(measuring dispersion tends to me less precise).

People are afraid of autofluorescence. Low AF is important in widefield,
critical in TIRF, but confocals don't really care.

My $0.02.
zdenek


On Mon, Feb 15, 2021 at 8:43 AM Michael Abanto <[hidden email]>
wrote:

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>
> just before that topic is closed... as I understand it the issue would be
> crystallization formed by mixing different oils on the same microscope
> objective. However, any one oil used without contamination of another would
> be fine. There might be subtle optimization for the optic/abberations that
> are brand specific and might only be noticed at high res.
>
>
> I have never done a test or seen the data for crystallization nor seen the
> optical differences published.
>
>
> Is it worth someone publishing a comparison of modern oils on different
> objectives. It would be great if we could all just buy Cargille for cheap
> (which I have heard that many companies do and then rebottle) and added
> specific chemicals as/if needed.
>
>
> ditto mounting media.
>
>
> Cheers,
>
>
> Mike
>
>
> Michael Abanto, PhD
> Head of Microscopy Facility
> Department of Biomedicine, Hebelstrasse 20
> University Hospital Basel
>
> tel [phone]
>
> http://biomedizin.unibas.ch/services/microscopy-facilities/facility-hebelstrasse/
> http://microscopynetwork.unibas.ch
> ________________________________
> From: Confocal Microscopy List <[hidden email]> on
> behalf of Khoroshyy Petro <[hidden email]>
> Sent: Monday, February 15, 2021 2:18:45 PM
> To: [hidden email]
> Subject: Re: Zeiss oil on Leica microscope
>
> *****
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>
> Thanks to everyone for their comments.
> While I think it is the same situation, but,  can anyone Any comments on
> the use of Immersol W  with non-Zeiss objectives.
> Best.
> Petro.
>
> On Thu, Feb 11, 2021 at 10:18 AM Antonio Virgilio Failla <[hidden email]>
> wrote:
>
> > On 10.02.2021 09:20, Khoroshyy Petro wrote:
> >
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
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> posting.
> > *****
> >
> > Hi, list.
> > I was told recently by a Leica engineer that he encountered many Leica
> > objectives destroyed by Zeiss oil. I was not able to figure out from him
> if
> > he was pooling my leg (which I think is the case) or was talking
> seriously.
> > Thanks.
> > Petro.
> >
> >
> > Hi Petro
> > is now 10 years that we are using Zeiss oil on Leica microscopes without
> > ojective damages and without the Leica service engineer
> >
> > <
> https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=&cad=rja&uact=8&ved=2ahUKEwi_5-TSv-HuAhWzQkEAHRTECf4QFjAAegQIBhAC&url=https%3A%2F%2Fen.wikipedia.org%2Fwiki%2FEngineer&usg=AOvVaw3sD6JZYnJ2ks0fC8YV0Lzk
> >complaining
> > about it.
> > Best wishes
> > Virgilio
> >
> > --
> > Dr. Antonio Virgilio Failla
> > Head of Light microscopy facility
> > Universitätsklinikum Hamburg-Eppendorf
> > UKE Microscopy Imaging Facility (UMIF)
> > Campus Forschung (N27), EG, Room 00.108.2
> > Martinistraße 52
> > 20246 Hamburg
> >
> > Tel.:  [phone]
> > Mobil: (+49) (0)152-22815841
> > Fax.:  [phone]
> >
> >
> > ------------------------------
> >
> > Universitätsklinikum Hamburg-Eppendorf; Körperschaft des öffentlichen
> > Rechts; Gerichtsstand: Hamburg | www.uke.de
> > Vorstandsmitglieder: Prof. Dr. Burkhard Göke (Vorsitzender), Joachim
> > Prölß, Prof. Dr. Blanche Schwappach-Pignataro, Marya Verdel
> > ------------------------------
> >
> > SAVE PAPER - THINK BEFORE PRINTING
> >
>
>
> --
> ______________________________
> Petro Khoroshyy
>

--
--
Zdenek Svindrych, Ph.D.
Research Scientist - Microscopy Imaging Specialist
Department of Biochemistry and Cell Biology
Geisel School of Medicine at Dartmouth