Re: Zeiss oil on Leica microscope

Posted by Steffen Dietzel on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Zeiss-oil-on-Leica-microscope-tp7591810p7591831.html

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Dear Mike,

I also would like to see such a  publication about it. What we have done
is to post some data on chromatic aberration on our web site, see here:
https://www.bioimaging.bmc.med.uni-muenchen.de/news/chrom-ab-100x/index.html

On our Leica SP8 STED, we checked chromatic aberration in reflection
mode, relative to 470 nm with the 100x1.4 STED white objective, which
was manufactured for particularly low chromatic aberration. With the
Zeiss 518F we get up to about 50 nm, with Cargille HF up to 100 nm and
more than that with the 775 nm depletion line. STED with 775 is
essentially not working with that oil, because the depletion donut is
too far off in z.

If you know that 'normal' NA 1.4 objectives may have an aberration of
 >200 nm anyway, if you are serious about it, you will have to correct
by post processing. and then it may not really matter which oil you use,
because they will differ just by the amount that you have to correct for.

If I get it right, the difference in behavior is described by the
dispersion value, which is given as ve= something.

We, hover, decided to stick to the 518F. Per slide, the additional costs
are negligible and we may save our users some trouble. And us the
trouble of users mixing different oils.

Best

Steffen

Am 15.02.2021 um 14:43 schrieb Michael Abanto:

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> just before that topic is closed... as I understand it the issue would be crystallization formed by mixing different oils on the same microscope objective. However, any one oil used without contamination of another would be fine. There might be subtle optimization for the optic/abberations that are brand specific and might only be noticed at high res.
>
>
> I have never done a test or seen the data for crystallization nor seen the optical differences published.
>
>
> Is it worth someone publishing a comparison of modern oils on different objectives. It would be great if we could all just buy Cargille for cheap (which I have heard that many companies do and then rebottle) and added specific chemicals as/if needed.
>
>
> ditto mounting media.
>
>
> Cheers,
>
>
> Mike
>
>
> Michael Abanto, PhD
> Head of Microscopy Facility
> Department of Biomedicine, Hebelstrasse 20
> University Hospital Basel
>
> tel [phone]
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> ________________________________
> From: Confocal Microscopy List <[hidden email]> on behalf of Khoroshyy Petro <[hidden email]>
> Sent: Monday, February 15, 2021 2:18:45 PM
> To: [hidden email]
> Subject: Re: Zeiss oil on Leica microscope
>
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> Thanks to everyone for their comments.
> While I think it is the same situation, but,  can anyone Any comments on
> the use of Immersol W  with non-Zeiss objectives.
> Best.
> Petro.
>
> On Thu, Feb 11, 2021 at 10:18 AM Antonio Virgilio Failla <[hidden email]>
> wrote:
>
>> On 10.02.2021 09:20, Khoroshyy Petro wrote:
>>
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>> Hi, list.
>> I was told recently by a Leica engineer that he encountered many Leica
>> objectives destroyed by Zeiss oil. I was not able to figure out from him if
>> he was pooling my leg (which I think is the case) or was talking seriously.
>> Thanks.
>> Petro.
>>
>>
>> Hi Petro
>> is now 10 years that we are using Zeiss oil on Leica microscopes without
>> ojective damages and without the Leica service engineer
>>
>> <https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=&cad=rja&uact=8&ved=2ahUKEwi_5-TSv-HuAhWzQkEAHRTECf4QFjAAegQIBhAC&url=https%3A%2F%2Fen.wikipedia.org%2Fwiki%2FEngineer&usg=AOvVaw3sD6JZYnJ2ks0fC8YV0Lzk>complaining
>> about it.
>> Best wishes
>> Virgilio
>>
>> --
>> Dr. Antonio Virgilio Failla
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>> Universitätsklinikum Hamburg-Eppendorf
>> UKE Microscopy Imaging Facility (UMIF)
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>> Martinistraße 52
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> --
> ______________________________
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