http://confocal-microscopy-list.275.s1.nabble.com/Zeiss-oil-on-Leica-microscope-tp7591810p7591833.html
A part the spherical aberration and the consequent losses in contrast and resolution, different oil have also different dispersion properties.
I had quite an unexpected surprise in the past when measuring chromatic shift on a DELTA VISION system first with Olympus and then with ZEISS oil.
D.
Av. Brasilia, Doca de Pedroucos
> On 15 Feb 2021, at 17:05, Cammer, Michael <
[hidden email]> wrote:
>
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> For most of our systems we use Cargill Labs type LDF.
>
> Unlike other brands, it does not dry into a sticky coating and provides consistency across the microscopes. We can jump from scope to scope. Our users don’t mix oils. None of our users have noticed any reduction in performance and when we've checked, we were hard pressed to measure differences ourselves.
>
> We have enough of a problem with people using Immersol W when they should be using 1.518 oil and vice versa.
>
> An exception is the Elyra where we use Zeiss oil. Another exception is Airyscan. And, of course, the silicon lenses, but we only have one scope with a subset of users.
>
> You can try different oils; just clean completely between them. You might find real performance improvements. And if I'm wrong about the type LDF, please let me know!
>
> Cheers-
>
> Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory
> NYU Langone Health, 540 First Avenue, SK2 Microscopy Suite, New York, NY 10016
> Office: 646-501-0567 Cell: 914-309-3270
[hidden email]
>
http://nyulmc.org/micros http://microscopynotes.com/>
>
>
>
>
> -----Original Message-----
> From: Confocal Microscopy List <
[hidden email]> On Behalf Of Steffen Dietzel
> Sent: Monday, February 15, 2021 11:55 AM
> To:
[hidden email]
> Subject: Re: Zeiss oil on Leica microscope
>
> [EXTERNAL]
>
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> To join, leave or search the confocal microscopy listserv, go to:
>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> Post images on
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> *****
>
> Dear Mike,
>
> I also would like to see such a publication about it. What we have done is to post some data on chromatic aberration on our web site, see here:
>
https://www.bioimaging.bmc.med.uni-muenchen.de/news/chrom-ab-100x/index.html>
> On our Leica SP8 STED, we checked chromatic aberration in reflection mode, relative to 470 nm with the 100x1.4 STED white objective, which was manufactured for particularly low chromatic aberration. With the Zeiss 518F we get up to about 50 nm, with Cargille HF up to 100 nm and more than that with the 775 nm depletion line. STED with 775 is essentially not working with that oil, because the depletion donut is too far off in z.
>
> If you know that 'normal' NA 1.4 objectives may have an aberration of
>> 200 nm anyway, if you are serious about it, you will have to correct by post processing. and then it may not really matter which oil you use, because they will differ just by the amount that you have to correct for.
>
> If I get it right, the difference in behavior is described by the dispersion value, which is given as ve= something.
>
> We, hover, decided to stick to the 518F. Per slide, the additional costs are negligible and we may save our users some trouble. And us the trouble of users mixing different oils.
>
> Best
>
> Steffen
>
> Am 15.02.2021 um 14:43 schrieb Michael Abanto:
>> *****
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>>
>> just before that topic is closed... as I understand it the issue would be crystallization formed by mixing different oils on the same microscope objective. However, any one oil used without contamination of another would be fine. There might be subtle optimization for the optic/abberations that are brand specific and might only be noticed at high res.
>>
>>
>> I have never done a test or seen the data for crystallization nor seen the optical differences published.
>>
>>
>> Is it worth someone publishing a comparison of modern oils on different objectives. It would be great if we could all just buy Cargille for cheap (which I have heard that many companies do and then rebottle) and added specific chemicals as/if needed.
>>
>>
>> ditto mounting media.
>>
>>
>> Cheers,
>>
>>
>> Mike
>>
>>
>> Michael Abanto, PhD
>> Head of Microscopy Facility
>> Department of Biomedicine, Hebelstrasse 20 University Hospital Basel
>>
>> tel 41 78 926 5316
>>
http://biomedizin.unibas.ch/services/microscopy-facilities/facility-he>> belstrasse/
>>
http://microscopynetwork.unibas.ch>> ________________________________
>> From: Confocal Microscopy List <
[hidden email]> on
>> behalf of Khoroshyy Petro <
[hidden email]>
>> Sent: Monday, February 15, 2021 2:18:45 PM
>> To:
[hidden email]
>> Subject: Re: Zeiss oil on Leica microscope
>>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>>
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http://www.imgur.com and include the link in your posting.
>> *****
>>
>> Thanks to everyone for their comments.
>> While I think it is the same situation, but, can anyone Any comments
>> on the use of Immersol W with non-Zeiss objectives.
>> Best.
>> Petro.
>>
>> On Thu, Feb 11, 2021 at 10:18 AM Antonio Virgilio Failla
>> <
[hidden email]>
>> wrote:
>>
>>> On 10.02.2021 09:20, Khoroshyy Petro wrote:
>>>
>>> *****
>>> To join, leave or search the confocal microscopy listserv, go
>>> to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy>>> Post images on
http://www.imgur.com and include the link in your posting.
>>> *****
>>>
>>> Hi, list.
>>> I was told recently by a Leica engineer that he encountered many
>>> Leica objectives destroyed by Zeiss oil. I was not able to figure out
>>> from him if he was pooling my leg (which I think is the case) or was talking seriously.
>>> Thanks.
>>> Petro.
>>>
>>>
>>> Hi Petro
>>> is now 10 years that we are using Zeiss oil on Leica microscopes
>>> without ojective damages and without the Leica service engineer
>>>
>>> <
https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=&cad=r>>> ja&uact=8&ved=2ahUKEwi_5-TSv-HuAhWzQkEAHRTECf4QFjAAegQIBhAC&url=https
>>> %3A%2F%2Fen.wikipedia.org%2Fwiki%2FEngineer&usg=AOvVaw3sD6JZYnJ2ks0fC
>>> 8YV0Lzk>complaining
>>> about it.
>>> Best wishes
>>> Virgilio
>>>
>>> --
>>> Dr. Antonio Virgilio Failla
>>> Head of Light microscopy facility
>>> Universitätsklinikum Hamburg-Eppendorf UKE Microscopy Imaging
>>> Facility (UMIF) Campus Forschung (N27), EG, Room 00.108.2
>>> Martinistraße 52
>>> 20246 Hamburg
>>>
>>> Tel.: (+49) 40-7410-51985
>>> Mobil: (+49) (0)152-22815841
>>> Fax.: (+49) 40-7410-40189
>>>
>>>
>>> ------------------------------
>>>
>>> Universitätsklinikum Hamburg-Eppendorf; Körperschaft des öffentlichen
>>> Rechts; Gerichtsstand: Hamburg | www.uke.de
>>> Vorstandsmitglieder: Prof. Dr. Burkhard Göke (Vorsitzender), Joachim
>>> Prölß, Prof. Dr. Blanche Schwappach-Pignataro, Marya Verdel
>>> ------------------------------
>>>
>>> SAVE PAPER - THINK BEFORE PRINTING
>>>
>>
>> --
>> ______________________________
>> Petro Khoroshyy
>
> --
> ------------------------------------------------------------
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> Ludwig-Maximilians-Universität München
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