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Cammer, Michael-2 on
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We use the system to image high speed transmission images, so for a sample that gives off its own light, I would expect you would turn off the light and use longer exposure times.
I would think you could put a sample on the stage, set the widefield transmission mode (no filters) and not turn the halogen lamp on, and collect with TV1 or TV2 depending which wavelengths you need. Or you could put in filters if you want.
Cheers-
Michael
-----Original Message-----
From: Confocal Microscopy List <
[hidden email]> On Behalf Of Michal Majkowski
Sent: Monday, March 8, 2021 9:32 AM
To:
[hidden email]
Subject: Re: Elyra 7 system for bioluminescence
[EXTERNAL]
Dear Michael,
I want to use Elyra Laser WF mode. In this mode structure for SIM is not used (as far as I understand SIM Elyra set-up). So a given laser illuminates a sample and photons are collected through an emission filter.
Of course I do not want to use laser as photons are generated by chemical reaction. Is it an answer for your question?
pon., 8 mar 2021 o 15:07 Cammer, Michael <
[hidden email]> napisał(a):
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> The structure for SIM is provided by the excitation light source. Does
> the emission in your system rely on this?
> Cheers-
> Michael Cammer
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> behalf of Michał Majkowski <
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> Dear List Members,
> I am just thinking about the use of our Elyra 7 system to visualise
> bioluminescence.
> Have any of you ever tried something similar? In systems dedicated for
> bioluminescence signal is detected with the same or similar camera
> (Andor iXon Ultra 897) though light path is less complex.
> My idea is to take time series with longest possible exposure time (1
> sec), convert T->Z and then sum up all slices. Does it have any sense?
> Thank you in advance
> Michal Majkowski
>
> Imaging Specialist
> University of Wroclaw
> Biotechnology Faculty
> Joliot-Curie 14a
> 50-383 Wroclaw POLAND
>
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