http://confocal-microscopy-list.275.s1.nabble.com/Elyra-7-system-for-bioluminescence-tp7591945p7591961.html
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> Hi Michal,
> you may want to use much longer exposure times (30 seconds for CMOS
> cameras, hours for CCDs) depending how weak your luminescence is. With
> EMCCDs, since the readout noise is negligible, you should get the same
> results by summing shorter exposure images, like you suggested. You may
> also bin the pixels for more SNR.
> For optimization and troubleshooting you can measure the luminescence from
> your cells with a suitable plate reader, they are much more sensitive.
> I haven't tried this with the Zeiss Axio stand, but one issue I found with
> the Nikon Ti-E and TE2000 is the stray light coming from internal
> components (probably the encoders). I had to power off the microscope body
> completely to get low background (I think Ti2-E can power off the encoders
> only, if needed).
> Also, dedicated systems may have much better light throughput, especially
> when you trade off resolution for collection efficiency (just compare the
> back focal plane aperture size of your microscope objective lens with let's
> say Nikkor 50 mm F/# 1.2.
> Good luck!
> Best, zdenek
>
> On Mon, Mar 8, 2021 at 8:50 AM Michał Majkowski <
>
[hidden email]> wrote:
>
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> > Dear List Members,
> > I am just thinking about the use of our Elyra 7 system to visualise
> > bioluminescence.
> > Have any of you ever tried something similar? In systems dedicated for
> > bioluminescence signal is detected with the same or similar camera (Andor
> > iXon Ultra 897) though light path is less complex.
> > My idea is to take time series with longest possible exposure time (1
> sec),
> > convert T->Z and then sum up all slices. Does it have any sense?
> > Thank you in advance
> > Michal Majkowski
> >
> > Imaging Specialist
> > University of Wroclaw
> > Biotechnology Faculty
> > Joliot-Curie 14a
> > 50-383 Wroclaw POLAND
> >
>
>
> --
> --
> Zdenek Svindrych, Ph.D.
> Research Scientist - Microscopy Imaging Specialist
> Department of Biochemistry and Cell Biology
> Geisel School of Medicine at Dartmouth
>