Posted by
Sathya Srinivasan on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Fluorescent-grid-in-z-slide-tp7591970p7591975.html
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Hi Charles,
We have an electronic microcrocater which measures the Z-axis in our
stereology system. The Z-axis measurement is very important for
stereological studies. There are a few published methods for Z axis
measurements:
-
https://www.agarscientific.com/media/import/03_Calibration_Standards_pgs_39-84_date_17_06_10_web.pdf(have different types of standards for X, Y and Z calibration including
beads of different sizes)
- Disector Z-axis mechanical method for stereology (
https://www.scielo.br/pdf/aabc/v82n2/28.pdf)
- A three-dimensional calibration device for the confocal microscope (
https://pdfs.semanticscholar.org/11ca/316460cfa41672f817388d0dbfa3d43f7cd8.pdf)
- Procedure for Calibrating the Z-axis of a Confocal Microscope-
Application for the Evaluation of Structured Surfaces (
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6387104/pdf/sensors-19-00527.pdf)
- Calibration of the automated Z-axis of a microscope using focus functions
(
https://d1rkab7tlqy5f1.cloudfront.net/TNW/Over%20faculteit/Decaan/Publications/1997/JoM97FBLVTY.pdf)
- Industrial Calibration Procedure for Confocal Microscopes (
https://www.mdpi.com/1996-1944/12/24/4137/pdf)
You can use your imagination with some fluorescence coating on surfaces at
different depths for your measurements. Not sure if all the links provided
above will work.
Good luck.
Sathya Srinivasan
ONPRC
On Thu, Mar 11, 2021 at 8:55 AM zhouzc <
[hidden email]> wrote:
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>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> Post images on
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>
> Hi all,
>
> I'm trying to measure how grin lenses distort and magnify the image in the
> z-axis. I know there are 2D fluorescent grids that are evenly spaced (eg.
>
https://www.psfcheck.com/about-us) for lateral measurements, but I was
> wondering if there any similar slides/samples but for axial measurements.
>
> Thanks!
> Charles
>