Experience/Recommendations with fast Piezo objective scanners

Posted by Nino Karpf on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Experience-Recommendations-with-fast-Piezo-objective-scanners-tp7592195.html

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Dear list,

can you recommend a piezo objective scanner?
I want to buy a fast (~10-20Hz sinusoidal @ 200µm range) piezo scanner to acquire multiphoton 3D volumes. Currently, I’m opting for the Thorlabs piezo system PFM450E. Objective will be either a Nikon CFI75 25X NA 1.1 / Olympus XLPLN25X NA 1.05 (or the like from Leica or Zeiss). So overall rather massive/heavy objectives.

So my questions are:
- Do you have experience with fast motion (>10Hz sinusoidal for 200µm range)? How is the movement? Any hysteresis?
- Do you prefer the Thorlabs system or a PI Pifoc (or similar system)? I’m opting for the Thorlabs system due to the resonant mode at up to 120Hz for 150g objectives.
- Can this be easily implemented into Labview/Micromanager/ICY etc. software environment?

Any recommendations or literature references are welcome!

Thank you,
Nino