Re: Experience/Recommendations with fast Piezo objective scanners

Posted by Benjamin Smith on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Experience-Recommendations-with-fast-Piezo-objective-scanners-tp7592195p7592196.html

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I'm not in the lab, so I don't know exactly what piezo-Z we have, but it
does drive the Olympus 20x/1.0NA dipping objective just fine with 30-50 µm
square wave jumps at 30 Hz.  The biggest thing is to make sure the driver
you choose has a position sensor output (ideally as an analog voltage).  As
you allude to, objectives have a fair amount of inertia so you need the
position feedback voltage to know when the objective actually arrives at
the new position so you can add a corresponding delay to the scan.  This
way, you can test your step sequence before you start imaging and record
the actual position as a function of time.  Then take the feedback curves
to determine how much time you'd like to wait before starting the next
scan.  For bonus points, you could readily implement this as a closed loop
feedback - meaning all the decisions happen without user input.

Also, as stated in Appendix D of the Thorlabs manual for the driver you
reference, the repeatability and hysteresis of a piezo driver changes with
temperature and age, and thus the manual also recommends using a position
sensor to resolve these issues.

On Tue, May 4, 2021 at 12:54 PM Sebastian 'Nino' Karpf <[hidden email]>
wrote:

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>
> Dear list,
>
> can you recommend a piezo objective scanner?
> I want to buy a fast (~10-20Hz sinusoidal @ 200µm range) piezo scanner to
> acquire multiphoton 3D volumes. Currently, I’m opting for the Thorlabs
> piezo system PFM450E. Objective will be either a Nikon CFI75 25X NA 1.1 /
> Olympus XLPLN25X NA 1.05 (or the like from Leica or Zeiss). So overall
> rather massive/heavy objectives.
>
> So my questions are:
> - Do you have experience with fast motion (>10Hz sinusoidal for 200µm
> range)? How is the movement? Any hysteresis?
> - Do you prefer the Thorlabs system or a PI Pifoc (or similar system)? I’m
> opting for the Thorlabs system due to the resonant mode at up to 120Hz for
> 150g objectives.
> - Can this be easily implemented into Labview/Micromanager/ICY etc.
> software environment?
>
> Any recommendations or literature references are welcome!
>
> Thank you,
> Nino

--
Benjamin E. Smith, Ph. D.
Imaging Specialist, Vision Science
University of California, Berkeley
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Berkeley, CA  94720-3200
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