Re: Experience/Recommendations with fast Piezo objective scanners

Posted by Nino Karpf on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Experience-Recommendations-with-fast-Piezo-objective-scanners-tp7592195p7592227.html

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Dear Ben,

I’m curious which piezo stage you’re using, since you report you have good results with it with a fairly heavy objective and fast movements. Do you have the exact model of your piezo stage?

Best,
Nino

> Am 04.05.2021 um 22:36 schrieb Benjamin Smith <[hidden email]>:
>
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>
> I'm not in the lab, so I don't know exactly what piezo-Z we have, but it
> does drive the Olympus 20x/1.0NA dipping objective just fine with 30-50 µm
> square wave jumps at 30 Hz.  The biggest thing is to make sure the driver
> you choose has a position sensor output (ideally as an analog voltage).  As
> you allude to, objectives have a fair amount of inertia so you need the
> position feedback voltage to know when the objective actually arrives at
> the new position so you can add a corresponding delay to the scan.  This
> way, you can test your step sequence before you start imaging and record
> the actual position as a function of time.  Then take the feedback curves
> to determine how much time you'd like to wait before starting the next
> scan.  For bonus points, you could readily implement this as a closed loop
> feedback - meaning all the decisions happen without user input.
>
> Also, as stated in Appendix D of the Thorlabs manual for the driver you
> reference, the repeatability and hysteresis of a piezo driver changes with
> temperature and age, and thus the manual also recommends using a position
> sensor to resolve these issues.
>
> On Tue, May 4, 2021 at 12:54 PM Sebastian 'Nino' Karpf <[hidden email]>
> wrote:
>
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>>
>> Dear list,
>>
>> can you recommend a piezo objective scanner?
>> I want to buy a fast (~10-20Hz sinusoidal @ 200µm range) piezo scanner to
>> acquire multiphoton 3D volumes. Currently, I’m opting for the Thorlabs
>> piezo system PFM450E. Objective will be either a Nikon CFI75 25X NA 1.1 /
>> Olympus XLPLN25X NA 1.05 (or the like from Leica or Zeiss). So overall
>> rather massive/heavy objectives.
>>
>> So my questions are:
>> - Do you have experience with fast motion (>10Hz sinusoidal for 200µm
>> range)? How is the movement? Any hysteresis?
>> - Do you prefer the Thorlabs system or a PI Pifoc (or similar system)? I’m
>> opting for the Thorlabs system due to the resonant mode at up to 120Hz for
>> 150g objectives.
>> - Can this be easily implemented into Labview/Micromanager/ICY etc.
>> software environment?
>>
>> Any recommendations or literature references are welcome!
>>
>> Thank you,
>> Nino
>
>
>
> --
> Benjamin E. Smith, Ph. D.
> Imaging Specialist, Vision Science
> University of California, Berkeley
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